Description
Product Information
| Product Name | Cat.No. | Spec. |
| 5 x SDS-PAGE Loading Buffer (Reduced) | G2013-5ML | 5mL |
| G2013-100ML | 100 mL |
Description
The 5 × SDS-PAGE Loading Buffer (Reduced), containing Tris-HCl buffer (63 mM, pH6.8), DTT (20 mM), glycerol (10%), SDS (2%) and bromophenol blue (0.0025%), is used for electrophoretic loading of reduced SDS-PAGE protein samples.
Storage and Handling Conditions
Product shipped at wet ice, stored at -20℃, valid for 12 months.
Component
| Component | G2013-5ML | G2013-100ML |
| 5× SDS-PAGE Loading Buffer (Reduced) | 5mL | 100 mL |
| Protocol | 1 | |
Assay Protocol / Procedures
1. Mix each 4 μL protein sample with 1 μL 5× SDS-PAGE Loading Buffer (reduced sample).
2. Heat in a metal bath or boiling water bath for 3-5 minutes to fully denature the protein sample.
3. It can be added to the spotting well after cooling to room temperature.
Note
1. The product should be returned to room temperature before used.
2. Please wear lab coat and disposable gloves during operation.
For Research Use Only!
Ver. No.: V2.0-202104
Product Information
| Product Name | Cat.No. | Spec. |
| 2 x SDS-PAGE Loading Buffer (Reduced) | G2031-1ML | 1 mL |
Description
The protein loading buffer is a two-fold concentrated protein loading buffer with bromophenol blue as dye, which is used for routine SDS-PAGE electrophoresis loading of protein samples. The main ingredients of this product include Tris-HCl buffer, glycerol, SDS, bromophenol blue and a small amount of reducing agent DTT.
Storage and Handling Conditions
Transport with wet ice; Store at -20℃, valid for 12 months.
Component
| Component | G2031-1ML |
| 2× SDS-PAGE Loading Buffer (Reduced) | 1 mL |
| Product Manual | |
Assay Protocol
1. The protein samples were thoroughly mixed with an equal volume ratio of 2 × protein loading buffer (reduced).
2. Heat in metal bath or boiling water bath for 3-5 min to fully denature the protein sample.
3. After cooling to room temperature, it can be added to the sample holes.
Note:
1. This product contains a small amount of the reducing agent DTT, has a slightly pungent odor, but does not contain mercaptoethanol. Please pay attention to protection when using.
2. Make sure the product is fully reheated before use.
3. For your safety and health, please wear a lab coat and disposable gloves when operating.
Product Informati
| Product Name | Cat.No. | Spec. |
| 2 x SDS-PAGE Loading Buffer (Reduced) | G2031-1ML | 1 mL |
Description
The protein loading buffer is a two-fold concentrated protein loading buffer with bromophenol blue as dye, which is used for routine SDS-PAGE electrophoresis loading of protein samples. The main ingredients of this product include Tris-HCl buffer, glycerol, SDS, bromophenol blue and a small amount of reducing agent DTT.
Storage and Handling Conditions
Transport with wet ice; Store at -20℃, valid for 12 months.
Component
| Component | G2031-1ML |
| 2× SDS-PAGE Loading Buffer (Reduced) | 1 mL |
| Product Manual | |
Assay Protocol
1. The protein samples were thoroughly mixed with an equal volume ratio of 2 × protein loading buffer (reduced).
2. Heat in metal bath or boiling water bath for 3-5 min to fully denature the protein sample.
3. After cooling to room temperature, it can be added to the sample holes.
Note:
1. This product contains a small amount of the reducing agent DTT, has a slightly pungent odor, but does not contain mercaptoethanol. Please pay attention to protection when using.
2. Make sure the product is fully reheated before use.
3. For your safety and health, please wear a lab coat and disposable gloves when operating.
