1. Recombinant Streptavidin (Freeze-Dried Powder), 5 mg $300

Product Information

Description

Recombinant Streptavidin is a recombinant biotin-binding protein derived from Streptomyces avidinii expressed by Escherichia coli and contains His tag. Streptavidin and biotin have very high affinity (Kd~10-15M) and low non-specific binding, so streptavidin-biotin system is widely used in various aspects of biological analysis. Streptavidin in its native state is a tetramer composed of four identical peptide chains with a molecular mass of about 65kDa. One streptavidin molecule can bind four biotin molecules. The recombinant streptavidin of this product has exactly the same properties as the natural streptavidin, and its purity is higher. It can not only be coupled with active substances such as enzyme and fluorescein, but also can be coupled with solid phase carriers such as magnetic beads and microspheres.

Source: E. coli strain carrying Streptomyces avidinii avidin gene;

Properties: white lyophilized powder

Definition of enzyme activity: The amount of enzyme required to bind 1 μg of biotin at room temperature is defined as 1 active unit U.

Purity and activity: purity ≥95% detected by SDS-PAGE; Protein activity ≥15 U/mg.

Storage and Handling Conditions

Transport with wet ice; Store at 4℃, valid for at least 12 months; Store at -20℃, valid for at least 24 months.

Component

Assay Protocol

Recombinant Streptavidin lyophilized powder resolution: Weigh appropriate amount of lyophilized powder, resolution with distilled water or deionized water, 2-8℃ for short-term storage. It is recommended to freeze at -20℃ or lower after packaging, which can be stored for a long time and avoid repeated freezing and thawing.

Note:

1. For your safety and health, please wear a lab coat and disposable gloves.

For Research Use Only!