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5. Odorless , 5×SDS-PAGE Protein Loading Buffer ,Odorless , 5ml $75
$75.00
In Stock
Description
Product IntroductionRelated Products
Product Information
| Product Name | Cat. No. | Spec. |
| 5×SDS-PAGE Protein Loading Buffer (Odorless, Reducing) | G2075-5ML | 5mL |
| G2075-100ML | 100 mL |
Product Introduction
5×SDS-PAGE Protein Loading Buffer(Odorless, Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins. The product contains bromophenol blue, which can confirm the progress of electrophoresis.
In addition, the product contains odorless reducing agent instead of dithiothreitol (DTT) or mercaptoethanol, making the protein loading operation safer and healthier, and the water solubility is more stable.
Storage and Handling Conditions
Storage at -20°C;Valid for 12 months.
Component
| Component | G2075-5ML | G2075–100ML |
| 5×SDS-PAGE Protein Loading Buffer (Odorless,Reducing) | 5 mL | 100 mL |
| Instruction Manual | One copy | |
Assay Protocol / Procedures
1. Allow the tube to reach room temperature and thoroughly mix before use;
2. Add it to the protein sample to make its working concentration 1×;
3. Fully denature the protein by heating in 95 ℃ water bath or metal bath for 10 min;
4. The samples can be loaded directly after reaching to room temperature;
5. Usually, electrophoresis can be stopped until the blue dye reaches the bottom of the gel.
Notes
1. The product must be completely dissolved before use, and stored at -20℃ as soon as possible after use.
2. Avoid direct contact between reagents and skin.
3. Please wear lab coat and disposable gloves during operation.
For Research Use Only!
version V2.0-202105
