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1. Recombinant Anti – beta Actin antibody ( Rabbit mAb ),100 μL $300
$300.00
In Stock
Description
Product Information
| Description | Rabbit monoclonal to beta Actin antibody |
| Protein full name | Actin, cytoplasmic 1 |
| Synonyms | ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta CytoskeletalActin, β-Actin |
| Immunogen | KLH conjugated synthetic peptide corresponding to Mouse beta-Actin |
| Isotype | IgG1 |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm |
| Predicted MW. | 42 kDa |
| Observed MW. | 45 kDa |
| Uniprot ID | P60710 |
Applications
| Applications | Species | Dilution | Positive Sample |
| WB | Human, Mouse, Rat | 1: 1000-1: 2000 | HeLa, Jurkat, MCF-7
PC-3, HepG2 |
| IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | spleen, colon cancer, lung, lung cancer |
| ICC/IF | Human, Mouse, Rat | 1: 300-1: 1000 | NIH/3T3, A549, etc. |
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Images
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Western blot analysis of beta-Actin (GB15003) at dilution of 1: 2000 |
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Immunohistochemistry of paraffin embedded mouse spleen using beta-Actin (GB15003) at dilution of 1: 1000 |
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Immunohistochemistry of paraffin embedded human colon cancer using beta-Actin (GB15003) at dilution of 1: 1000 |
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Immunohistochemistry of paraffin embedded human lung cancer using beta-Actin (GB15003) at dilution of 1: 1000 |
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Immunohistochemistry of paraffin embedded rat lung using beta-Actin (GB15003) at dilution of 1: 1000 |
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Immunocytochemistry analysis of 4% paraformaldehyde fixed NIH/3T3 cells using β-Actin (GB15003) at dilution of 1: 1000 |
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Immunocytochemistry analysis of 4% paraformaldehyde fixed HeLa cells using β-Actin (GB15003) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
