1. New Decalcification Solution (Quick Decalcification); 500mlx2 $ 83.9

Product Information

Description

In pathological experiments, when sections are made for bone tissue or some calcium-containing tissues, they cannot be made directly because of their high hardness, so they need to remove the calcium to make the tissues soft before they can be made into sections. Commonly used decalcification reagents such as EDTA, organic acid, inorganic acid, the EDTA is a relatively mild chelating calcium supplements, the organizational structure affect the minimum, some enzymes can better save organization, organization of EDTA decalcified immunohistochemical and in situ hybridization detection can be done, the decalcified slowly, decalcified cycle is long, generally take several weeks to months, And the size of decalcified tissue. Organic acid is the use of acid dissolved calcium, decalcification speed is relatively fast, and there is a certain degree of damage to tissue structure. The inorganic acid decalcification is usually hydrochloric acid or nitric acid decalcification, which has the fastest decalcification speed but the largest tissue damage.

The new decalcification solution (fast decalcification) of this product is acid decalcification solution, the main active ingredient is formic acid.

Storage and Handling Conditions

Store and transport at room temperature; It is valid for 12 months.

Assay Protocol

Place the tissue to be decalcified into the original decalcified solution. The volume of decalcified solution should be 15-20 times the volume of the tissue. During decalcification, change the decalcification solution every two days, check the degree of decalcification every day, until the tissue becomes soft, the pin can be smoothly inserted into the tissue, and the hand touching the bone tissue is elastic and can easily bend, which is judged as the end of decalcification. The decalcification time of small tissues is about two days, and the decalcification time of large tissues is about a week, and the time of different tissues varies. During decalcification, observe the tissue closely to avoid excessive decalcification damage to the tissue and influence the subsequent experiment.

Note:

1. This product is acidic and volatile. Please use it in a well-ventilated place with good protection to avoid inhalation.

2. Pay close attention to tissue decalcification during decalcification to avoid excessive decalcification affecting subsequent experiments.

3. This product may destroy proteins in tissues, and EDTA is recommended for decalcification if subsequent antigen immunodetection is required.

4. Wear a lab coat and disposable gloves during operation.

   For Research Use Only!