9. Ham’s F-12K, GlutaPlus, 500 ml $40 (Ham’s F-12K,no L-Glutamine, G4561-500ML, $40 )

SKU: G4560-500ML
$45.00
In Stock
SKU: G4560-500ML Categories: ,

Description

Please click here to see the video. thanks

and another video to see the product quality. 

Product Information

Product Name Cat. No. Spec.
Ham’s F-12K, GlutaPlus G4560-500ML 500 mL

 


Product Description/Introduction

Ham’s F-12K medium is a Kaighn’s improved version of Ham’s F-12 nutrient mix, originally designed for culturing primary human hepatocytes and differentiated mouse and chicken cells under low serum conditions. Ham’s F-12K improves the amino acid and sodium pyruvate concentration, reduces the glucose content, and modifies the composition and content of inorganic salts based on the original Ham’s F-12.Compared to other medium, Ham’s F-12K contains many unique components such as putrescine, thymine, hypoxanthine, and zinc, in addition to a higher content of sodium pyruvate.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add serum or serum-free additives depending on the cell type.

This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 7.0 mM D-Glucose, 2.0 mM L-Alanyl-L-Glutamine, 2.0 mM sodium pyruvate and phenol red indicator, without HEPES.

 


Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

 

For Research Use Only!

G4560 Hams F-12K Formula

Components Concentration Components Concentration
mg/L mM mg/L mM
Amino acids Inorganic salts
Glycine 15 0.2 Calcium Chloride (CaCl2)(anhyd.) 102 0.9189
L-Alanine 18 0.2022 Copper Sulfate Pentahydrate (CuSO4·5H2O) 0.002 0.0000080
L-Arginine hydrochloride 422 2.0 Iron Sulfate Heptahydrate (FeSO4·5H2O) 0.8 0.002878
L-Asparagine-H2O 30 0.2 Magnesium Chloride (MgCl2)(anhyd.) 49.7 0.5231
L-Aspartic Acid 26.6 0.2 Magnesium Sulfate (MgSO4) (anhyd.) 192 1.600
L-Cysteine hydrochloride-H2O 70 0.3977 Potassium Chloride (KCl) 285 3.8000
L-Glutamic Acid 29 0.1973 Sodium Bicarbonate (NaHCO3) 2500 29.76
L-Alanyl-L-Glutamine 434.4 2 Sodium Chloride (NaCl) 7530 129.9
L-Histidine hydrochloride-H2O 45.8 0.2181 Dibasic sodium phosphate (Na2HPO4) (anhyd.) 115.5 0.813
L-Isoleucine 7.88 0.06015 Sodium dihydrogen Phosphate monobasic (NaH2PO4) (anhyd.) 59.0 0.492
L-Leucine 26.2 0.2 Zinc Sulfate Heptahydrate (ZnSO4·7H2O) 0.144 0.0005
L-Lysine hydrochloride 73 0.3989 Other components
L-Methionine 8.96 0.06013 D-Glucose (Dextrose) 1260 7
L-Phenylalanine 9.92 0.06012 Hypoxanthine 4 0.03053
L-Proline 69 0.6 Alphalipoic Acid 0.21 0.001019
L-Serine 21 0.2 Phenol red 3 0.007970
L-Threonine 23 0.1933 Diaminobutane dihydrochloride 0.32 0.001987
L-Tryptophan 4.1 0.02010 Sodium pyruvate 220 2.0
L-Tyrosine Disodium Salt 13.5 0.05153 Thymine 0.7 0.002892
L-Valine 23 0.1966
Vitamins
Biotin 0.07 0.0002869
Choline chloride 14 0.10
D-Calcium pantothenate 0.5

 

0.001048
Folic Acid 1.3 0.002948
Niacinamide 0.037 0.0003033
Pyridoxine hydrochloride 0.06 0.0002913
Riboflavin 0.04 0.0001064
Thiamine hydrochloride 0.3 0.0008902
Vitamin B12 1.4 0.001033
i-Inositol 18 0.10
Product recommendation
Cat.No.
Product Name
Spec.
Operation
G4560-500ML
Ham’s F-12K, GlutaPlus
500 mL
G4561-500ML
Ham’s F-12K, no L-Glutamine
500 mL

Shopping Cart
No products in the cart.