9. Antibody Elution Buffer (Regular), 100 mL $100

SKU: G2078-100ML
$100.00
In Stock
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SKU: G2078-100ML Category:

Description

Product Information

Product Name Cat. No. Spec.
Antibody Elution Buffer G2078-100ML 100 mL

Product Description

Antibody Elution Buffer provides a robust but gentle method for removing primary and secondary antibodies from Western blots that were detected with chemiluminescent substrates. Antibody Elution Buffer (Plus) can be used with both nitrocellulose and PVDF-probed membranes.

Cost-effective: Save valuable time and samples; strip blots effectively the first time.

Quick and efficient: Incubate for 5-15 minutes at room temperature to strip antibody.

OdorlessThiol-free, no offensive odors.

Ready and easy to useNo dilution necessary


Storage and Shipping Conditions

Ship with wet ice, store at 2-8℃, valid for 12 months.


Components

Component G2078-100ML
Antibody Elution Buffer 100 mL
Manual 1 pc

Assay Protocol

1. Wash blot once for 5 minutes in TBST to remove the chemiluminescent substrate.

2. Add sufficient Antibody Elution Buffer to cover the blot and incubate for 5 to 15 minutes at room temperature with gentle shaking.

3. Remove the blot from the Antibody Elution Buffer and wash 3 times for 5 minutes each in TBST with gentle shaking.

4. Block the membrane in blocking buffer before continuing with the second immunoprobing experiment.


Note

1.  This product is corrosive, take caution when using it.

2. Reuse is not recommended.

3. The membrane will become transparent when in use and will return to normal after cleaning.

4.  For your safety and health, please wear safety glasses, gloves, or protective clothing.

Table 1: Reference for antibody elution buffer selection

Product Features G2078 G2079
pH Strong acid Strong acid
Effect of Protein Reserve on The Membrane Excellent Good
Effect of Antibody Stipping Good Excellent
Membrane NC and PVDF NC and PVDF
Condition for Select primary antibody is susceptible to Elution Buffer removing high-affinity primary antibodies

 

For Research Use Only!

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