9. Anti -beta Actin Mouse mAb, 100ul $299

SKU: GB12001-100
$299.00
In Stock
SKU: GB12001-100 Category:

Description

Product Information

Protein full name Actin, cytoplasmic 1
Synonyms ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse β-Actin
Uniprot ID P60710
Isotype IgG1,κ
Purity Affinity purification
Subcellular location Cytoplasm
Predicted MW. / Observed MW. 42 kDa / 45 kDa

Applications

WB Human, Mouse, Rat 1: 1000-1: 2000 spleen, kidney, brain
IHC/IF Human, Mouse, Rat 1: 400-1: 2000 colon, kidney, liver
ICC/IF Human, Mouse, Rat 1: 300-1: 1000 NIH/3T3, A549

Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.


Images

Picture 1.png Western blot analysis of beta Actin (GB12001) at dilution of 1: 1000
Picture 2.png Western blot analysis of beta Actin (GB12001) at dilution of 1: 1000
Picture 3.png Immunohistochemistry of paraffin embedded human colon using actin (GB12001) at dilution of 1: 1000 (200x lens)
Picture 4.png Immunohistochemistry of paraffin embedded rat liver using actin (GB12001) at dilution of 1: 1000 (200x lens)
Picture 5.png Immunohistochemistry of paraffin embedded mouse stomach using actin (GB12001) at dilution of 1: 1000 (400x lens)
Picture 6.png Immunohistochemistry of paraffin embedded rat kidney using actin (GB12001) at dilution of 1: 1000 (400x lens)
Picture 7.png Immunohistochemistry of paraffin embedded human liver cancer using actin (GB12001) at dilution of 1: 1000 (200x lens)
Immunocytochemistry analysis of 4% paraformaldehyde fixed NIH/3T3 cells using β-Actin (GB12001) at dilution of 1: 1000
Immunocytochemistry analysis of 4% paraformaldehyde fixed HeLa cells using β-Actin (GB12001) at dilution of 1: 1000

Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

Product recommendation
Cat.No.
Product Name
Spec.
Operation
G2030-1ML
5 x SDS-PAGE Loading Buffer (Non-Reduced)
1 mL

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