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9. Anti -beta Actin Mouse mAb, 100ul $299
$299.00
In Stock
Description
Product Information
Protein full name | Actin, cytoplasmic 1 |
Synonyms | ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse β-Actin |
Uniprot ID | P60710 |
Isotype | IgG1,κ |
Purity | Affinity purification |
Subcellular location | Cytoplasm |
Predicted MW. / Observed MW. | 42 kDa / 45 kDa |
Applications
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | spleen, kidney, brain |
IHC/IF | Human, Mouse, Rat | 1: 400-1: 2000 | colon, kidney, liver |
ICC/IF | Human, Mouse, Rat | 1: 300-1: 1000 | NIH/3T3, A549 |
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Images
Western blot analysis of beta Actin (GB12001) at dilution of 1: 1000 | |
Western blot analysis of beta Actin (GB12001) at dilution of 1: 1000 | |
Immunohistochemistry of paraffin embedded human colon using actin (GB12001) at dilution of 1: 1000 (200x lens) | |
Immunohistochemistry of paraffin embedded rat liver using actin (GB12001) at dilution of 1: 1000 (200x lens) | |
Immunohistochemistry of paraffin embedded mouse stomach using actin (GB12001) at dilution of 1: 1000 (400x lens) | |
Immunohistochemistry of paraffin embedded rat kidney using actin (GB12001) at dilution of 1: 1000 (400x lens) | |
Immunohistochemistry of paraffin embedded human liver cancer using actin (GB12001) at dilution of 1: 1000 (200x lens) | |
Immunocytochemistry analysis of 4% paraformaldehyde fixed NIH/3T3 cells using β-Actin (GB12001) at dilution of 1: 1000 | |
Immunocytochemistry analysis of 4% paraformaldehyde fixed HeLa cells using β-Actin (GB12001) at dilution of 1: 1000 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
Product recommendation
Cat.No.
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Product Name
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Spec.
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Operation
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G2030-1ML
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5 x SDS-PAGE Loading Buffer (Non-Reduced)
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1 mL
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