8. TRITC-labeled Phalloidin (Red Fluorescence)

Product Information

Product Name: TRITC-labeled Phalloidin (Red Fluorescence) Product Number: G1041-100T Size: 100 T

Product Description

Phalloidin, also known as Phalloidin toxin, is a bicyclic peptide initially isolated from the toxic mushroom Amanita phalloides. It exhibits a high affinity and specificity for binding to F-actin, a component of muscle fibers, with no binding to G-actin. Phalloidin demonstrates similar affinities for different fiber sizes, and its binding ratio typically involves one phalloidin molecule per actin subunit. Phalloidin has minimal non-specific binding, resulting in distinct staining of both stained and unstained regions. It can stain F-actin at nanomolar concentrations, making it useful for labeling, identifying, and quantifying F-actin distribution.

This product is TRITC-labeled Phalloidin provided in a 20 μM storage solution format with DMSO as the solvent. The recommended working concentration range is 80-200 nM, using 200 μL of working solution per staining, resulting in a 100 nM working concentration. This product allows for 100 cell staining procedures.

Storage and Transportation

Stored at -20°C in the dark and shipped with ice packs (wet ice). Shelf life is 12 months.

Components

• G1041-100T TRITC-labeled Phalloidin (Red Fluorescence) 100 T
• Instruction Manual 1 copy

Preparation Before Experiment

Prepare 1× PBS buffer (pH 7.2-7.4), BSA, fixative solution, permeabilization solution, anti-fade mounting medium, and ready-to-use DAPI staining solution.

For the first use of this product, thaw the product thoroughly and centrifuge at low speed for 1 minute to prevent liquid loss along the tube wall. It is recommended to aliquot based on the amount needed for a single experiment to prevent solvent evaporation loss. Store at -20°C in the dark.

Before the formal experiment, prepare a 1% BSA-containing PBS solution. Mix 1 μL of TRITC-labeled Phalloidin with 199 μL of 1% BSA-containing PBS to obtain a 100 nM working solution. The commonly used working concentration is 80-200 nM, with 100 nM recommended. The working solution of TRITC-labeled Phalloidin can be used on the same day, stored at room temperature in the dark.

Experimental Procedure

[Follow the same steps as provided in the previous translation, replacing “FITC” with “TRITC” where appropriate.]

Notes

[Follow the same notes as provided in the previous translation.]

This product is for research purposes only and not intended for clinical diagnosis.

[The note about product packaging upgrade is also included.]