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8. Recombinant Anti- GAPDH antibody ( Rabbit mAb ); 100 μL $199
$199.00
In Stock
Description
Product Information
| Protein full name | Glyceraldehyde-3-phosphate dehydrogenase |
| Synonyms | GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse GAPDH |
| Uniprot ID | P16858 |
| Isotype | IgG1 |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm, Nucleus, Membrane |
| Predicted MW. / Observed MW. | 36 kDa / 36 kDa |
Applications
| WB | Human, Mouse, Rat | 1: 1000-1: 2000 | lung, brain, etc. |
| IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | kidney, testis, etc. |
| ICC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | HeLa |
Background
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed.
Images
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Western blot analysis of GAPDH (GB15004) at dilution of 1: 2000 |
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Immunohistochemistry of paraffin embedded mouse kidney using GAPDH (GB15004) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded mouse testis using GAPDH (GB15004) at dilution of 1: 500 |
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Immunocytochemistry analysis of 4% paraformaldehyde fixed HeLa cells using GAPDH (GB15004) at dilution of 1: 500 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
