8. Ham’s F-12, no L-Glutamine, 500 ml $40

SKU: G4563-500ML
$45.00
In Stock
SKU: G4563-500ML Categories: ,

Description

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Product Information

Product Name Cat. No. Spec.
Ham’s F-12, no L-Glutamine G4563-500ML 500 mL

 


Product Description/Introduction

Ham’s F-12 nutriment Mixture is based on Ham’s F-10 nutriment mixture, and it is originally used for serum-free culture of CHO cells. Ham’s F-12 is often used as the basic medium for serum-free culture, especially suitable for single cell culture and clonal culture at low serum levels. It is also widely used in the culture of tumor cells and primary cells after adding serum, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myoblasts and chicken embryonic cells. In addition, Ham’s F-12 can be mixed with DMEM in equal volumes to give a more nutritious DMEM/F-12 medium.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.

This product is sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 10 mM D-glucose, 1.0 mM sodium pyruvate, phenol red indicator, no HEPES buffer system and L-glutamine.

 


Storage and Shipping Conditions

Ship at room temperature; Store at 2-8℃, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves, or protective clothing.

 

For Research Use Only!

Ham’s F-12 medium, no L-Glutamine Formula

Components Concentration Components Concentration
mg/L mM mg/L mM
Amino acids Inorganic salts
Glycine 7.507 0.1 Potassium Chloride (KCl) 223.6 2.98
L-Alanine 8.90 0.10 Sodium bicarbonate(NaHCO3) 1176 14
L-Arginine monohydrochloride 210.66 1.0 Sodium chloride (NaCl) 7599 131.02
L-Asparagine monohydrate 15.02 0.10 Sodium Phosphate dibasic (Na2HPO4) (anhyd.) 141.96 1.0
L-Asparagine 13.31 0.1 Calcium Chloride (CaCl2)(anhyd.) 33.22 0.30
L-Cysteine hydrochloride hydrate 35.05 0.20 Cupric Sulfate (CuSO4·5H2O) 0.0025 0.00001
L-Glutamic acid 14.71 0.1 Ferrous Sulfate (FeSO4·7H2O) 0.834 0.003
L-Glutamine Magnesium chloride (MgCl2)(anhyd.) 57.35 0.60
L-Histidine hydrochloride monohydrate 20.96 0.1 Zinc Sulfate(ZnSO4·7H2O) 0.862 0.003
L-Isoleucine 4.005 0.031 Other components
L-Leucine 13.12 0.1 D-Glucose (Dextrose) 1802.0 10.01
L-Lysinehydrochloride 36.50 0.199 Hypoxanthine 1.13 0.00319
L-Methionine 4.51 0.03 Lipoic acid 110 1.0
L-Phenylalanine 5.01 0.03 Phenol Red 4.083 0.03
L-Proline 34.5 0.3 Putrescine 2HCl 0.084 0.0003
L-Serine 10.5 0.1 Sodium pyruvate 0.210 0.00102
L-Threonine 11.9 0.10 Thymidine 0.16107 0.001
L-Tryptophan 2.0 0.01 D-Glucose (Dextrose) 0.701 0.00289
L-Tyrosine disodium salt 7.8 0.0298
L-Valine 11.7 0.1
Vitamins
Biotin 0.0073 0.0000299
Choline chloride 14 0.1
D-Calcium pantothenate 0.50 0.001048
Folic Acid 1.30 0.002948
Niacinamide 0.04 0.000295
Pyridoxine hydrochloride 0.06 0.000291
Riboflavin 0.04 0.000098
Thiamine hydrochloride 0.30 0.000890
Vitamin B12 1.40 0.001033
i-Inositol 18 0.1

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