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7. SweScript All-in-One RT SuperMix for qPCR (One-Step gDNA Remover), 50 rxns $460
$460.00
In Stock
Description
Product Information
| Product Name | Cat.No. | Spec. |
| SweScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Remover) | G3337-50 | 50 rxns |
| G3337-100 | 100 rxns |
Description/Introduction
This product contains 5×Ready-to-use master mix (5×SweScript All-in-One SuperMix for qPCR) and gDNA remover reagents, can remove genomic remnants of the template while the reverse transcription reaction is performed.The kit contained 5×SweScript All-in-One No-RT Control SuperMix as a reverse-transcriptase-free control to determine whether the qPCR template was derived from cDNA.The master mix contains all reagents required for the reverse transcription reaction(SweScript RT, RNase Inhibitor, Random Primer, Oligo(dT)18 Primer, dNTPs and the optimized buffer system). SweScript RT is a mutant reverse transcriptase obtained by in vitro evolutionary screening and is characterized by high thermal stability and processivity. gDNA Remover contains a thermosensitive DNase that is 30 times more active than DNase I and acts only on dsDNA and is susceptible to inactivation under high temperature conditions.
Storage and Handling Conditions
Wet ice bag transportation; Stored at -20℃ temperature, valid for 12 months.
Component
| Component Number | Component | G3333-50 | G3333-100 |
| G3337-1 | 5×SweScript All-in-One SuperMix for qPCR | 200 μl | 400 μL |
| G3337-2 | gDNA Remover | 50 μL | 100 μL |
| G3337-3 | Nuclease-Free Water | 1 mL | 1 mL |
| G3337-4 | 5×SweScript All-in-One No-RT Control SuperMix for qPCR | 20 μL | 40 μL |
| Manual | 1 pc | 1 pc | |
Assay Protocol / Procedures
1.Synthesis of the first strand cDNA and Genome removal
(1) Preparation reaction system (recommended 20ul reaction system) :
| Component | Volume |
| 5×SweScript All-in-One SuperMix for qPCR | 4 μL |
| gDNA Remover | 1 μL |
| Total RNA/mRNA | 0.1 ng-5ug / 10 pg-0.5ug |
| Nuclease-Free Water | Add to 20 μL |
(2) Gently mix and centrifuge;
(3) Reverse transcription program setting:
| Temperature | Time |
| 25℃ | 5 min |
| 42℃ | 15-30 min |
| 85℃ | 5 s |
Note:
1. Please wear disposable gloves during operation to avoid RNase contamination.
2. Be sure to place 5×SweScript All-in-One SuperMix、5×SweScript All-in-One No-RT Control SuperMix、gDNA Remover on ice.
3. 5×SweScript SuperMix、5×SweScript All-in-One No-RT Control SuperMix、gDNA Remover contains high concentrations of glycerol and should be briefly centrifuged before use.
4. For high GC or complex templates, the reverse transcription temperature can be raised to 65℃.
5. If a negative control is required, just replace the equal volume of G3337-1 5×SweScript All-in-One SuperMix for qPCR in the reaction system with G3337-4 5×SweScript All-in-One No-RT Control SuperMix for qPCR.
6. The reverse transcription products can be stored at -20℃ for a short time. If long-term storage is needed, it is recommended to store them at -80℃ after packing to avoid repeated freeze-thaw cycles.
7. If the subsequent qPCR primers are designed across exons, the genome removal step can be omitted.
For Research Use Only!
Ver. No.: V1.0-202112
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