7. Recombinant Anti -GAPDH antibody ( Mouse mAb ); 100 μL $199

SKU: GB15002-100.
$199.00
In Stock
SKU: GB15002-100. Category:

Description

Product Information

Description Mouse monoclonal to GAPDH
Protein full name Glyceraldehyde-3-phosphate dehydrogenase
Synonyms GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase
Immunogen KLH conjugated synthetic peptide corresponding to Mouse GAPDH
Isotype IgG1
Purity Affinity purification
Subcellular location Cytoplasm, Nucleus, Membrane
Predicted MW. 36kDa
Observed MW. 36kDa
Uniprot ID P16858

Applications

WB Human, Mouse, Rat 1: 1000-1: 2000 heart, lung, liver, kidney, brain
IHC/IF Human, Mouse, Rat 1: 500-1: 1000 breast cancer, esophageal cancer, kidney

Background

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.


Images

图片 1.png WB analysis of GAPDH (GB15002).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.

图片 2.png IHC analysis of GAPDH (GB15002).

Sample: Human breast carcinoma (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.

图片 3.png IHC analysis of GAPDH (GB15002).

Sample: Human esophageal cancer (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.

图片 5.png IHC analysis of GAPDH (GB15002).

Sample: Mouse kindey (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.

图片 9.png IHC analysis of GAPDH (GB15002).

Sample: Rat kindey (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.

图片 10.png IHC analysis of GAPDH (GB15002).

Sample: Mouse testis (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.

图片 11.png IHC analysis of GAPDH (GB15002).

Sample: Rat spinal cord (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.


Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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