7. Ham’s F-12, GlutaPlus, 500 ml $40

SKU: G4562-500ML
$45.00
In Stock
SKU: G4562-500ML Categories: ,

Description

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Product Information

Product Name Cat. No. Spec.
Ham’s F-12, GlutaPlus G4562-500ML 500 mL

 


Product Description/Introduction

Ham’s F-12 (Ham’s F-12 Nutrient Mixture) is based on Ham’s F-10 nutrient mix and was originally designed for serum-free culture of CHO cells. Ham’s F-12 is often used as a basal culture medium for serum-free cultures and is particularly suitable for single cell cultures and clonogenic cultures at low serum levels. It is also widely used in the culture of tumor cells and primary cells by adding serum, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myogenic cells, chicken embryonic cells, etc. In addition, Ham’s F-12 is mixed with DMEM in equal volume to obtain a more nutrient-rich DMEM/F-12 medium.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add serum or serum-free additives depending on the cell type.

This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 10 mM D-Glucose, 1.0 mM L-Alanyl-L-Glutamine, 1.0 mM sodium pyruvate and phenol red indicator, without HEPES.

 


Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

 

For Research Use Only!

G4562 Hams F-12 Formula

Components Concentration Components Concentration
mg/L mM mg/L mM
Amino acids Inorganic salts
Glycine 7.507 0.1 Potassium Chloride (KCl) 223.6 2.98
L-Alanine 8.90 0.1 Sodium Bicarbonate (NaHCO3) 1176 14
L-Arginine hydrochloride 210.66 1.0 Sodium Chloride (NaCl) 7599 131.02
L-Asparagine-H2O 15.02 0.1 Dibasic sodium phosphate (Na2HPO4) (anhyd.) 141.96 1.0
L-Aspartic Acid 13.31 0.1 Calcium Chloride (CaCl2)(anhyd.) 33.22 0.30
L-Cysteine hydrochloride-H2O 35.05 0.20 Copper Sulfate Pentahydrate (CuSO4·5H2O) 0.0025 0.00001
L-Glutamic Acid 14.71 0.1 Iron Sulfate Heptahydrate (FeSO4·5H2O) 0.834 0.003
L-Alanyl-L-Glutamine 217.2 1.0 Magnesium Chloride (MgCl2)(anhyd.) 57.35 0.60
L-Histidine hydrochloride-H2O 20.96 0.1 Zinc Sulfate Heptahydrate (ZnSO4·7H2O) 0.862 0.003
L-Isoleucine 4.005 0.031 Other components
L-Leucine 13.12 0.1 D-Glucose (Dextrose) 1802.0 10.01
L-Lysine hydrochloride 36.5 0.199 Phenol red 1.13 0.00319
L-Methionine 4.5 0.03 Sodium pyruvate 110 1.0
L-Phenylalanine 5.0 0.03 Hypoxanthine 4.083 0.03
L-Proline 34.5 0.3 Linoleic Acid 0.084 0.0003
L-Serine 10.5 0.1 Alphalipoic Acid 0.210 0.00102
L-Threonine 11.9 0.10 Diaminobutane dihydrochloride 0.161 0.001
L-Tryptophan 2.0 0.01 Thymidine 0.701 0.00289
L-Tyrosine Disodium Salt 7.8 0.0298
L-Valine 11.7 0.1
Vitamins
Biotin 0.0073 0.0000299
Choline chloride 14 0.1
D-Calcium pantothenate 0.50

 

0.001048
Folic Acid 1.30 0.002948
Niacinamide 0.04 0.000295
Pyridoxine hydrochloride 0.06 0.000291
Riboflavin 0.04 0.000098
Thiamine hydrochloride 0.30 0.000890
Vitamin B12 1.40 0.001033
i-Inositol 18 0.1
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