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6. SweScript First Strand cDNA Synthesis SuperMix for PCR, 50 rxns $460
$460.00
In Stock
Description
Product Information
| Product Name | Cat.No. | Spec. |
| SweScript First Strand cDNA Synthesis SuperMix for PCR | G3336-50 | 50 rxns |
Description
This product includes a 5× ready-to-use premix (5×SweScript First Strand cDNA Synthesis SuperMix for PCR) specially developed for first-strand cDNA Synthesis. Simply add template RNA and nuclease-free Water. The premixed solution contained all the reagents required for the reverse transcription reaction (SweScript RT, RNase Inhibitor, Oligo(dT)18 Primer, Random Primer, dNTPs, and optimized buffer system). After adjusting the use ratio of Oligo(dT)18 Primer and Random Primer, combined with the mutant SweScript RT reverse transcriptase with high thermal stability and strong continuous synthesis ability obtained through in vitro evolutionary screening, at least 15 KB of first-strand cDNA can be synthesized in the range of 42℃ to 55℃. The synthesized cDNA product can be directly used for subsequent PCR reaction, which is easy to operate and reduces the probability of contamination.
Storage and Handling Conditions
Wet ice transportation; Store at -20℃ for 12 months.
Component
| Component Number | Component | G3336-50 |
| G3336-1 | 5×SweScript First Strand cDNA Synthesis SuperMix for PCR | 200 μL |
| G3336-2 | Nuclease-Free Water | 1 mL |
| Product Maunal | 1 | |
Assay Protocol
(1) Preparation of reverse transcription reaction system (20 μL reaction system is recommended)；
| Component | Volume |
| 5×SweScript First Strand cDNA Synthesis SuperMix for PCR | 4 μL |
| Total RNA/ mRNA | 0.1 ng-5 μg / 10 pg-0.5 μg |
| Nuclease-Free Water | Add to 20 μL |
(2) Mix gently and centrifuge；
(3) Reverse transcription program Settings：
| Temperature | Time |
| 25℃ | 5 min |
| 42-50℃a | 15-30 min |
| 85℃ | 5 s |
a: For high GC or complex templates, the reverse transcription temperature can be increased to 55 ° C.
Note:
1. Wear disposable gloves during operation to avoid RNase contamination.
2. Be sure to place 5×SweScript First-Strand cDNA Synthsis SuperMix for PCR on ice.
3. 5×SweScript First-Strand cDNA Synthsis SuperMix for PCR contains high concentrations of glycerol and should be briefly centrifuged before use.
4. Reverse transcription products can be stored at -20℃ for a short time. If long-term storage is required, it is recommended to store at -80℃ after packaging to avoid repeated freezing and thawing.
5. If the subsequent PCR primers are designed across introns, the genome removal step can be selected.
For Research Use Only!
Ver. No.: V1.0-202111
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