6. Anti -GAPDH Rabbit pAb, 100ul $300

SKU: GB11002-100
$300.00
In Stock
SKU: GB11002-100 Category: Tag:

Description

 

Product Information

Description GAPDH rabbit polyclonal antibody
Protein full name Glyceraldehyde-3-phosphate dehydrogenase
Synonyms GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse GAPDH
Isotype IgG
Purity Affinity purification
Subcellular location Cytoplasm, Nucleus, Membrane
Predicted MW. 36 kDa
Observed MW. 36 kDa
Uniprot ID P16858

Applications

WB Human, Mouse, Rat 1: 1000-1: 2000 brain, spleen, kidney
IHC/IF Human, Mouse, Rat 1: 500-1: 1000 kidney, testis

Background

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed.


Images

01 GB11002_GAPDH_WB.jpg WB analysis of GAPDH (GB11002).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

02 GB11002_GAPDH_WB.jpg WB analysis of GAPDH (GB11002).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

03 GB11002_20220309_GAPDH_GB11001 M kindey d500_20x_IHC(1).jpg IHC analysis of GAPDH (GB11002).

Sample: Mouse kindey (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

04 GB11002_.20220309_GAPDH_GB11001 R testis d500_20x_IHC.jpg IHC analysis of GAPDH (GB11002).

Sample: Rat testis (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.


Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

Product recommendation
Cat.No.
Product Name
Spec.
Operation
G2031-1ML
2 x SDS-PAGE Loading Buffer (Reduced)
1 mL

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