6. 10×TBST Buffer Solution, 1 L $79

Product Information

Product Description/Introduction

TBST buffer (Tris-buffered saline with Tween-20 detergent) is an isotonic buffer salt solution used in biology. It consists of Tris-HCl to form a stable pH buffer system, NaCl to provide isotonic conditions and Tween-20 as a decontaminant to increase the elution capacity of the buffer. It can be applied in Western Blot experiments to wash away reagents such as non-specific binding antibodies on membranes, as well as in immunofluorescence and immunohistochemistry experiments for the preparation of the blocking solution, the preparation of primary or secondary antibodies, and the washing of primary or secondary antibodies after incubation to reduce the background and enhance the signal-to-noise ratio. The product is a 10-fold concentrate solution which consists of 100 mM Tris, 1.5 M NaCl, 1% Tween-20. Should be diluted 10-fold before use.

Storage and Shipping Conditions

Ship and store at room temperature; Valid for 12 months.

Assay Protocol/Procedures

Mix 100 mL of this product with 900 mL of deionised or distilled water to obtain 1 x TBST solution containing 10 mM Tris, 150 mM NaCl, 0.1% Tween-20, pH 7.2-7.6 at 25°C.

Note

1. Once diluted to 1 x working solution, this product needs to be stored at 4°C and used up within one month. If stored at room temperature, use within one week is recommended.

2. Place the product in 37°C water bath until crystals completely dissolved, it will not affect the use.

3. For your safty and health, please wear safety glasses, gloves, or protective clothing.