54. M199, GlutaPlus

SKU: G4620-500ML
$40.00
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Description

Product Information

Product Name Cat. No. Spec.
M199, GlutaPlus G4620-500ML 500 mL

 


Product Description/Introduction

M199, known as Medium 199, was originally designed for the culture of chicken embryo fibroblasts and is now widely used for the culture of various animal cells, including non-mammalian cells. Compared to other basal medium, M199 contains a number of unique components, including adenine, adenosine, hypoxanthine, thymine and other vitamins. Different balanced salt solution can be used, for example Earle’s Balanced Salts Solution for CO2 environments and Hank’s Balanced Salts Solution for non-CO2 environments. M199 is especially suitable for the culture of non-transformed cells and is also commonly used for virology, rat pancreatic epithelial cells and mouse lens tissue.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.

This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains Earle’s Balanced Salts Solution, 1000 mg/L D-glucose, L-alanyl-L-glutamine.

 


Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

 

For Research Use Only!

G4620 M199 Formula

 

Components Concentration Components Concentration
mg/L mM mg/L mM
Amino acids Inorganic salts
Glycine 50.0 0.6667 Calcium Chloride (CaCl2)(anhyd.) 200.0 1.802
L-Alanine 25.0 0.2809 Ferric Nitrate (Fe(NO3)3·9H2O) 0.7 0.001733
L-Arginine hydrochloride 70.0 0.3318 Magnesium Sulfate (MgSO4) (anhyd.) 97.7 0.8139
L-Asparagine acid 30.0 0.2256 Potassium Chloride (KCl) 400.0 5.333
L-Cysteine hydrochloride·H2O 0.10 0.0006 Sodium Bicarbonate(NaHCO3) 2200.0 26.190
L-Cystine 2HCl 33.9 0.1083 Sodium chloride (NaCl) 6800.0 117.24
L-Glutamic acid 75.0 0.5102 Sodium dihydrogen phosphate(NaH2PO4)(anhyd.) 121.7 1.014
L-Alanyl-L-Glutamine 148.8 0.6849 Other components
L-Histidine hydrochloride·H2O 21.9 0.1042 D-Glucose (Dextrose) 1000.0 5.556
L-Hydroxyproline 10.0 0.0763 Phenol Red 18.83 0.053135
L-Isoleucine 40.0 0.3053 Cholesterol 0.20 0.00051680
L-Leucine 60.0 0.4580 Glutathione (reduced) 0.05 0.00016287
L-Lysine hydrochloride 70.0 0.3825 Sodium Acetate 50.0 0.60976
L-Methionine 15.0 0.1007 Tween 80 20
L-Phenylalanine 25.0 0.1515 Hypoxanthine Na 0.40 0.0029412
L-Proline 40.0 0.3478 2-deoxy-D-ribose 0.50 0.003731
L-Serine 25.0 0.2381 Adenine sulfate 5.772 0.024752
L-Threonine 30.0 0.2521 Adenosine 5’-phosphate 0.211 0.0005764
L-Tryptophan 10.0 0.0490 Adenosine 5’-triphosphate 0.911 0.0016529
L-Tyrosine disodium salt dihydrate 58.0 0.2222 Guanine hydrochloride 0.30 0.00159574
L-Valine 25.0 0.2137 Ribose 0.50 0.0033333
Vitamins Thymine 0.30 0.0023810
Ascorbic Acid 0.050 0.0002841 Uracil 0.30 0.002679
Biotin 0.010 0.0000410 Xanthine-Na 0.34 0.002237
Choline chloride 0.50 0.003571
D-Calcium pantothenate 0.010 0.0000210
Folic Acid 0.010 0.0000227
Menadione (Vitamin K3) 0.01606 0.00005814
Niacinamide 0.0250 0.0002049
Nicotinic Acid (Niacin) 0.0250 0.0002033
Para-Aminobenzoic Acid 0.0500 0.0003650
Pyridoxal hydrochloride 0.0250 0.0001225
Pyridoxine hydrochloride 0.0250 0.0001214
Riboflavin 0.0100 0.00002660
Thiamine hydrochloride 0.0100 0.00002967
Vitamin A (acetate) 0.100 0.00030488
Vitamin D2 (Calciferol) 0.100 0.00025189
Alpha Tocopherol phos. Na salt 0.010 0.0000180
I-lnositol 0.050 0.0002778

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