55. IMDM, GlutaPlus

SKU: G4640-500ML
$40.00
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Description

Product Information

Product Name Cat. No. Spec.
IMDM (Iscove’s Modified Dulbecco’s Medium),GlutaPlus G4640-500ML 500 mL

 


Product Description/Introduction

IMDM, known as Iscove’s Modified Dulbecco Medium, is Iscove’s modified DMEM. It was originally designed for the culture of erythroid precursor cell and macrophages. Based on DMEM, IMDM is added with trace element selenium, additional amino acids and vitamins, potassium nitrate instead of iron nitrate, which is nutrient-rich, ideal for rapid proliferation and high-density cell culture, such as Mouse B Lymphocytes cell, LPS Stimulated B cell, Bone Marrow Hematopoietic Stem cell, T-lymphocyte and various hybridoma cells. In addition, IMEM is also used as a base fluid for a number of unique serum-free medium.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add serum or serum-free additives depending on the cell type.

This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains L-Alanyl-L-Glutamine, sodium pyruvate, phenol red indicator and HEPES. Please check the official website for the specific recipe.

 


Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

 

For Research Use Only!

G4640 IMDM Formula

Components Concentration Components Concentration
mg/L mM mg/L mM
Amino acids Inorganic salts
Glycine 30.0 0.40 Calcium Chloride (CaCl2)(anhyd.) 165.0 1.4865
L-Alanine 25.0 0.2809 Magnesium Sulfate (MgSO4) (anhyd.) 97.67 0.8139
L-Arginine hydrochloride 84.0 0.3981 Potassium Chloride (KCl) 330.0 4.40
L-Asparagine 25.0 0.1894 Potassium Nitrate (KNO3) 0.076 0.0007520
L-Aspartic Acid 30.0 0.2256 Sodium Bicarbonate (NaHCO3) 3024.0 36.0
L-Cystine 2HCl 91.4 0.2920 Sodium Chloride (NaCl) 4505.0 77.67
L-Glutamic Acid 75.0 0.5102 Sodium Phosphate monobasic (NaH2PO4) (anhyd.) 108.7 0.9058
L-Alanyl-L-Glutamine 868.9 4.00 Sodium selenite (Na2SeO3) 0.0170 0.0000983
L-Histidine hydrochloride-H2O 42.0 0.20 Other components
L-Isoleucine 105.0 0.8015 D-Glucose (Dextrose) 4500.0 25.0
L-Leucine 105.0 0.8015 HEPES 5958.0 25.03
L-Lysine hydrochloride 146.0 0.7978 Phenol red 14.107 0.0399
L-Methionine 30.0 0.2013 Sodium pyruvate 110.000 1.0
L-Phenylalanine 66.0 0.40
L-Proline 40.0 0.3478
L-Serine 42.0 0.40
L-Threonine 95.0 0.7983
L-Tryptophan 16.0 0.0784
L-Tyrosine Disodium Salt 104.0 0.4622
L-Valine 94.0 0.8034
Vitamins
Biotin 0.0130 0.00005330
Choline chloride 4.0 0.02857
D-Calcium pantothenate 4.0 0.00839
Folic Acid 4.0 0.009070
Niacinamide 4.0 0.032787
Pyridoxine hydrochloride 4.0 0.019608
Riboflavin 0.40 0.001064
Thiamine hydrochloride 4.0 0.01187
Vitamin B12 0.0130 0.00000959
i-Inositol 7.2 0.040
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