53. Leibovitz’s L-15, GlutaPlus

SKU: G4570-500ML
$40.00
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Description

Product Content

Name Cat No. Size
Leibovitz’s L-15 G4570-500ML 500 mL

 


Product Description

Leibovitz’s L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO2 equilibration.

Leibovitz’s L-15 Medium contains no proteins, lipids, or growth factors. Therefore, Leibovitz’s L-15 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

All raw material components used for Servicebio cell culture media products are screened through using strict quality control testing. Servicebio cell culture media are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of cell culture media are subjected to sterility test, pH, osmolality, endotoxin and cell culture test.

The major character of this Leibovitz’s L-15 Medium is as follows:

l With 5.0 mM Galactose, 2.05 mM L-glutamine, 5.0 mM Sodium Pyruvate, with phenol red.

l Without Glucose, Sodium Bicarbonate and HEPES.

The complete formulation is available.

 


Storage

Storage conditions: 2℃ to 8℃, protect from light

Shipping conditions: Ambient

Shelf life: 12 months from date of manufacture

 

For Research Use Only!

 

G4570 Leibovitz’s L-15 Formula

mg/L mM mg/L mM
Amino Acids Other Components
Glycine 200.2 2.6667 D(+)Galactose 900 5.00
L-Alanine 225.0 2.5281 Phenol Red 9.41 0.02657
L-Arginine HCl 605.3 2.8736 Sodium Pyruvate 550 5.00
L-Asparagine H2O 284.3 1.8939
L-Cysteine 174.2 0.9917
L-Glutamine 300.3 2.0548
L-Histidine 250.0 1.6129
L-Isoleucine 250.3 1.9084
L-Leucine 125.2 0.9542
L-Lysine HCl 94.0 0.5137
L-Methionine 75.1 0.5034
L-Phenylalanine 125.1 0.7576
L-Serine 200.2 1.9048
L-Threonine 300.3 2.5210
L-Tryptophan 20.0 0.09804
L-Tyrosine Disodium Salt 372.3 1.6548
L-Valine 100.0 0.8547
Vitamins
Choline chloride 1.00 0.007143
D-Calcium pantothenate 1.00 0.002096
Folic Acid 1.00 0.002268
Niacinamide 1.00 0.008197
Pyridoxine hydrochloride 1.00 0.004854
Riboflavin 5′-phosphate Na 0.10 0.000209
Thiamine monophosphate 0.94 0.002262
i-Inositol 2.00 0.011111
Inorganic Salts
Magnesium Sulfate (MgSO4) (anhyd.) 97.7 0.8139
Potassium Chloride (KCl) 400.0 5.3333
Sodium Chloride (NaCl) 8000.0 137.93
Sodium Phosphate dibasic (Na2HPO4) anhydrous 189.9 1.3380
Calcium Chloride (CaCl2) (anhyd.) 140.0 1.2613
Potassium Phosphate monobasic (KH2PO4) 60.0 0.4412
Magnesium Chloride (anhydrous) 93.7 0.9863

 

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