50. DMEM/F-12, no L-Glutamine, no HEPES

SKU: G4611-500ML
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Description

Product Information

Product Name Cat. No. Spec.
DMEM/F-12, no L-Glutamine, no HEPES G4611-500ML 500 mL

 


Product Description/Introduction

DMEM/F-12 medium was modified by mixing DMEM with Ham’s F-12 medium at a ratio of 1:1. The modified DMEM/F-12 medium is richer in nutrients and contains more micronutrients, which is a widely used basal medium for supporting the growth of many different mammalian cells, as well as for the culture of mammalian cells at low serum levels and for clonal density culture. DMEM/F-12 has also been used as a base medium for the development of serum-free media.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.

This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains 17.5 mM D-glucose, 0.5 mM sodium pyruvate, phenol red indicator, no L-glutamine and HEPES buffer system.

Ship at room temperature; Store at 2-8℃, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves, or protective clothing.

 

For Research Use Only!

DMEM/F-12 Medium

 

Components Concentration Components Concentration
mg/L mM mg/L mM
Amino acids Inorganic salts
Glycine 18.75 0.250 Calcium Chloride (CaCl2)(anhyd.) 116.6 1.050
L-Alanine 4.45 0.050 Cupric Sulfate (CuSO4·5H2O) 0.0013 0.0000052
L-Arginine monohydrochloride 147.5 0.699 Ferric Nitrate (Fe(NO3)3·9H2O) 0.05 0.0001238
L-Asparagine monohydrate 7.5 0.050 Ferrous Sulfate (FeSO4·7H2O) 0.417 0.0015
L-Asparagine 6.65 0.050 Magnesium chloride (MgCl2)(anhyd.) 28.64 0.301
L-Cysteine hydrochloride hydrate 17.56 0.100 Magnesium sulfate (MgSO4) (anhyd.) 48.84 0.407
L-Cystine dihydrochloride 31.29 0.100 Potassium Chloride (KCl) 311.8 4.157
L-Glutamic acid 7.35 0.050 Sodium bicarbonate(NaHCO3) 2438 29.024
L-Glutamine Sodium chloride (NaCl) 6995.5 120.6
L-Histidine hydrochloride monohydrate 31.48 0.150 Disodium (hydrogen) phosphate(Na2HPO4)(anhyd.) 71.02 0.500
L-Isoleucine 54.47 0.416 Sodium dihydrogen phosphate(NaH2PO4)(anhyd.) 54.34 0.453
L-Leucine 59.05 0.451 Zinc Sulfate(ZnSO4·7H2O) 0.432 0.0015
L-Lysinehydrochloride 91.25 0.499 Other components
L-Methionine 17.24 0.116 D-Glucose (Dextrose) 3151 17.51
L-Phenylalanine 35.48 0.215 Hypoxanthine Na 2.39 0.015
L-Proline 17.25 0.150 Linoleic Acid 0.042 0.00015
L-Serine 26.25 0.250 Lipoic acid 0.105 0.00051
L-Threonine 53.45 0.449 Phenol Red 8.1 0.022
L-Tryptophan 9.02 0.044 Putrescine dihydrochloride 0.081 0.00050
L-Tyrosine disodium salt 55.79 0.214 Thymidine 0.365 0.00151
L-Valine 52.85 0.452 Sodium pyruvate 55 0.500
Vitamins HEPES
Biotin 0.0035 0.0000143
Choline chloride 8.98 0.0641
D-Calcium pantothenate 2.24 0.0047
Folic Acid 2.65 0.0060
Niacinamide 2.02 0.0166
Pyridoxine hydrochloride 2.013 0.0098
Riboflavin 0.219 0.0006
Thiamine hydrochloride 2.17 0.0064
Vitamin B12 0.68 0.0005
i-Inositol 12.6 0.07
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