5. Trypan Blue Stain,

Product Information

Description

Trypan Blue is a reactive dye for cells, which is often used to detect the integrity of cell membranes. Because the envelope of normal living cells is complete, it can repel trypan blue, making it unable to enter the cells, while the permeability of dead cells or cells with incomplete envelope increases, and can be dyed blue by trypan blue. Therefore, the cell survival rate can be determined simply and quickly by trypan blue staining.

This product is trypan blue dye solution with a concentration of 0.4%, which is used for living cell dyeing and counting.

Storage and Handling Conditions

Wet ice transportation; Store at 4 ℃ for 12 months.

Component

Assay Protocol

1. The adherent cells were digested by trypsin to prepare single cell suspension and diluted appropriately.

2. Staining: Mix the cell suspension with trypan blue dye at a volume ratio of 9:1, and dye for 3-5 min.

3. Observe under the microscope, or take 10 μ L was added to the cell counting plate, and the cell counter was used for automatic counting. The dead cells were dyed obviously blue, and the living cells were refused to be dyed colorless and transparent.

4. Counting: count living cells and dead cells respectively within three minutes.

5. Statistics of cell vitality: living cell rate (%)=number of living cells/(number of living cells+number of dead cells) × 100%。

For Research Use Only!