5. Ponceau S Staining, 100 ml $248

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Product Information

Description

Ponceau S staining solution can be used for the rapid detection of proteins on PVDF membrane, nitrocellulose membrane (NC membrane) and cellulose acetate membrane. The principle is that Ponceau S is negatively charged and can bind to positively charged amino acid residues, while Ponceau S can also bind to non-polar regions of the protein, resulting in a red band. The staining of protein by Ponceau S is reversible and can be washed off with distilled water, PBS or other appropriate solution.

The main ingredient of this product is 0.2% Ponceau S.

Storage and Handling Conditions

Store and transport at room temperature, valid for 12 months.

Component

Assay Protocol

1. The PVDF membrane, nitrocellulose membrane or cellulose acetate membrane were immersed in Ponceau S working solution, and the staining was shaken for 3-5 min or longer until clear bands appeared.

2. Rinse the stained film in distilled water for a few seconds to slightly remove the background color until the background is light and the protein band is clear.

3. Rinse with distilled water, PBS or other appropriate solution 2-3 times, 3-5 min each time to remove Ponceau S, and conduct the subsequent Western experiment.

Note:

1. Ponceau S staining solution can be recycled and used repeatedly.

2. Ponceau S staining solution is not suitable for protein staining detection on nylon membrane.

3. For your safety and health, please wear a lab coat and disposable gloves when operating.

For Research Use Only!