5. Plus : Mycoplasma Elimination Reagent Plus (100×): 5 ml (100 time concentration) $310

Introduction

This product is a combination of quinolones, tetracyclines and macrolides, which inhibit the DNA replication and protein synthesis of mycoplasma, so as to effectively remove mycoplasma. This product can completely remove mycoplasma from infected cells within 5-7 days. After changing the normal medium, the cells are in good condition, and there is no resungization phenomenon in continuous multi-generation culture.

Storage and Handling Conditions

Transport with wet ice; Store at -20℃, avoid repeated freezing and thawing, valid for 12 months.

Components

Usage

1. Add 1 mL of Mycoplasma Elimination Reagent Plus (100×) to 100 mL of cell medium (containing serum, without double antibody) and mix thoroughly to obtain medium containing Mycoplasma Elimination Reagent.

2. When Mycoplasma infected cells are subcultured or liquid is changed, the medium containing Mycoplasma Elimination Reagent Plus is changed for routine cell culture.

3. After one week of culture, some cells were seeded into 6-well plates (or other standard culture vessels) and cultured with normal complete medium. The remaining cells were continued to be cultured with medium containing Mycoplasma Elimination Reagent Plus.

4. Cells in 6-well plates were cultured with normal complete medium for 2-3 days, and mycoplasma detection was carried out when the confluence of cells reached more than 90% (G1900, G1901, G1902 are recommended).

5. According to the test result, perform the following operations:

A) Positive result: continue to repeat steps 3-4 until the test result is negative.

B) Negative results: the cell group containing Mycoplasma Elimination Reagent Plus medium was replaced with normal complete medium, and Mycoplasma detection was performed after a period of culture; A negative retest indicates that the clearance is complete.

Note

1. This product has been tested in a variety of mycoplasma infected cell lines, but different cell lines have different sensitivity to reagents. If the cytotoxicity is too great or the removal effect is not good, the solubility of the product can be adjusted appropriately, and other scavenger can be selected (G4007, G4008 are recommended).

2. There are many ways for cells to be infected with Mycoplasma. It is recommended to add mycoplasma preventive reagent (G4009 is recommended) in daily culture.

3. For cells that have been identified as mycoplasma contamination, separate ultra-clean tables should be used as far as possible to avoid cross-contamination.

4. For your safety and health, please wear a lab coat and disposable gloves when operating.

Attached Table: Features of Mycoplasma Removal Series

For Research Use Only!