5. L-Glutamine (200 mM)

Product Information

Product Description

L-Glutamine is an essential nutrient for cell culture, a source of cell growth capacity, and is also involved in protein synthesis and nucleic acid metabolism. L-Glutamine deficiency can lead to poor cell growth and even death. L-Glutamine is unstable in solution and is prone to spontaneous degradation with time, temperature, pH, etc. When the cells show poor growth, the culture medium can be supplemented with a certain L-Glutamine to meet the needs of cell growth.

Storage and Shipping Conditions

Ship with wet ice; Store at 2-8℃, valid for 24 months. It is recommended to divide into smaller portions and store at -20℃.

Product Contents

Assay Protocol

This product is a 200 mM stock solution with 0.85% NaCl as solvent and is filtered and sterilized by 0.22 μm filter membrane. The recommended working concentration in the culture medium is 1-4 mM, the amount of addition varies according to the needs of the cells, add to the culture medium in proportion.For example, if the desired final concentration in the medium is 2 mM, add 1 mL of this product per 100 mL of medium.

Note

1. This product is filtered and sterilized by 0.22 μm filter membrane, need aseptic operation when using to avoid contamination.

2. This product is a 200 mM concentrate, please dilute as needed.

3. Thaw in 2-8℃, shake well and use. Avoid repeated freezing and thawing, recommend freezing and storing after dispensing.

4. For your safety and health, please wear safety glasses, gloves or protective clothing.

For Research Use Only!