7. BCECF, AM Fluorescence pH Assay Kit, $799

Description

BCECF, AM Fluorescence pH Assay Kit

Product Information:

• Product Name: BCECF, AM Fluorescence pH Assay Kit
• Product Number: G1726-100T
• Specification: 100 tests
• Price: ¥300

Product Description:

The BCECF, AM Fluorescence pH Assay Kit enables precise and quantitative measurement of intracellular pH using the fluorescent probe BCECF, AM. BCECF, AM is a cell-permeable dye that exhibits changes in fluorescence intensity in response to alterations in pH. This assay kit offers researchers a robust tool to track and study intracellular pH fluctuations in live cells, allowing deeper insights into various cellular processes and functions.

The kit includes BCECF, AM as the core probe, along with specialized buffer solutions and comprehensive instructions, facilitating accurate determination of intracellular pH levels in biological specimens. The emitted fluorescence signal correlates with the pH of the intracellular milieu, enabling real-time monitoring of pH dynamics.

Storage and Transportation:

• Shipped with dry ice
• Store at -20°C in a dark and dry environment
• Shelf life: 12 months

Kit Components:

Note: The stated number of tests corresponds to the 96-well plate assay system.

Operating Instructions:

1. Prepare your cell or tissue samples according to the specific requirements of your experiment.
2. Prepare the BCECF, AM Working Solution: Follow the instructions provided in the product manual to prepare the BCECF, AM Working Solution.
3. Staining and Labeling:

   3.1. Remove the existing cell culture medium and wash the cells or tissue samples with PBS or an appropriate buffer.

   3.2. Discard the wash buffer and add the prepared BCECF, AM Working Solution. Incubate the samples in the dark at room temperature or 37°C for the designated time.

   3.3. After the incubation period, wash the samples with PBS or buffer to remove excess probe.

4. Fluorescence Detection: Employ suitable instruments such as fluorescence microscopes, microplate readers, flow cytometers, etc., to quantify changes in intracellular pH by monitoring alterations in the fluorescence intensity of BCECF, AM.

Note:

1. The recommended staining conditions and incubation times may vary based on the cell type and experimental requirements. Adjustments can be made as needed.
2. Take precautions to minimize light exposure during the staining and detection procedure to prevent potential interference with fluorescence signals.
3. Adhere to laboratory safety practices by wearing appropriate protective attire and gloves.
4. This product is exclusively intended for research purposes and is not suitable for clinical diagnosis or treatment.