5. Anti -GAPDH Mouse mAb; 100 μL $199

SKU: GB12002-100.
$199.00
In Stock
SKU: GB12002-100. Category:

Description

Product Information

Protein full name Glyceraldehyde-3-phosphate dehydrogenase
Synonyms GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse GAPDH
Uniprot ID P16858
Isotype IgG1,κ
Purity Affinity purification
Subcellular location Cytoplasm, Nucleus, Membrane
Predicted MW. / Observed MW. 37 kDa / 37 kDa

Applications

WB Human, Mouse, Rat 1: 500-1: 1000 spleen, lung, kidney
IHC/IF Human, Mouse, Rat 1: 200-1: 400 kidney, stomach, testis

Background

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed.


Images

01 GB12002_GAPDH_WB.jpg WB analysis of GAPDH (GB12002).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.

02 GB12002_GAPDH_WB-2.jpg WB analysis of GAPDH (GB12002).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.

03 GB12002_4D6D10_GAPDH_M testis d200_20.0x_IHC.jpg IHC analysis of GAPDH (GB12002).

Sample: Mouse testis (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 200, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.

04 GB12002_4D6D10_GAPDH_R kidney d200_20.0x_IHC.jpg IHC analysis of GAPDH (GB12002).

Sample: Rat kidney (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 200, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.

05 GB12002_GAPDH_H stomach d200_20.0x_IHC.jpg IHC analysis of GAPDH (GB12002).

Sample: Human stomach (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 200, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.

06 GB12002_20220309_GAPDH_GB12001 H liver cancer d1000_ 20x_IHC.jpg IHC analysis of GAPDH (GB12002).

Sample: Human liver cancer (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.

07 GB12002_20220309_GAPDH_GB12001 R spleen d1000_ 20x_IHC.jpg IHC analysis of GAPDH (GB12002).

Sample: Rat spleen (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.


Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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