49. Ham’s F-12K, no L-Glutamine

SKU: G4561-500ML
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Description

Product Information

Product Name Cat. No. Spec.
Ham’s F-12K, no L-Glutamine G4561-500ML 500 mL

 


Product Description/Introduction

Ham’s F-12K (Kaighn’s) Medium is a modification of Ham’s F-12 Nutrient Mixture. Ham’s F-12K (Kaighn’s) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment. Ham’s F-12K improves the concentration of amino acid and pyruvate, reduces the content of glucose, and modifies the composition and content of inorganic salts on the basis of the original Ham’s F-12. Compared with other media, Ham’s F-12K contains many unique components, such as putrescine, thymidine, hypoxanthine, zinc, in addition to a higher content of sodium pyruvate.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, no protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.

This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains 7.0 mM D-glucose, 2.0 mM sodium pyruvate, phenol red indicator, no HEPES buffer system and L-glutamine.

 


Storage and Shipping Conditions

Ship at room temperature; Store at 2-8℃, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves, or protective clothing.

 

For Research Use Only!

Ham’s F-12K medium, no L-Glutamine Formula

Components Concentration Components Concentration
mg/L mM mg/L mM
Amino acids Inorganic salts
Glycine 15 0.2 Calcium Chloride (CaCl2)(anhyd.) 102 0.9189
L-Alanine 18 0.2022 Cupric Sulfate (CuSO4·5H2O) 0.002 0.0000080
L-Arginine monohydrochloride 422 2.0 Ferrous Sulfate (FeSO4·7H2O) 0.8 0.002878
L-Asparagine monohydrate 30 0.2 Magnesium chloride (MgCl2)(anhyd.) 49.7 0.5231
L-Asparagine 26.6 0.2 Magnesium sulfate(MgSO4)(anhyd.) 192 1.600
L-Cysteine hydrochloride hydrate 70 0.3977 Potassium Chloride (KCl) 285 3.800
L-Glutamic acid 29 0.1973 Sodium bicarbonate(NaHCO3) 2500 29.76
L-Glutamine Sodium chloride (NaCl) 7530 129.9
L-Histidine hydrochloride monohydrate 45.8 0.2181 Sodium Phosphate dibasic (Na2HPO4) (anhyd.) 115.5 0.813
L-Isoleucine 7.88 0.06015 Sodium Phosphate monobasic (NaH2PO4) (anhyd.) 59.0 0.492
L-Leucine 26.2 0.2 Zinc Sulfate(ZnSO4·7H2O) 0.144 0.0005
L-Lysinehydrochloride 73 0.3989 Other components
L-Methionine 8.96 0.06013 D-Glucose (Dextrose) 1260 7
L-Phenylalanine 9.92 0.06012 Hypoxanthine 4 0.03053
L-Proline 69 0.6 Lipoic acid 0.21 0.001019
L-Serine 21 0.2 Phenol Red 3 0.007970
L-Threonine 23 0.1933 Putrescine 2HCl 0.32 0.001987
L-Tryptophan 4.1 0.02010 Sodium pyruvate 220 2.0
L-Tyrosine disodium salt 13.5 0.05153 Thymidine 0.7 0.002892
L-Valine 23 0.1966
Vitamins
Biotin 0.0073 0.0000299
Choline chloride 14 0.1
D-Calcium pantothenate 0.50 0.001048
Folic Acid 1.30 0.002948
Niacinamide 0.04 0.000295
Pyridoxine hydrochloride 0.06 0.000291
Riboflavin 0.04 0.000098
Thiamine hydrochloride 0.30 0.000890
Vitamin B12 1.40 0.001033
i-Inositol 18 0.1
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