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47. RPMI-1640 medium, no L-Glutamine, no calcium
$40.00
In Stock
Description
| Product Information | |
|---|---|
| Product Name | |
| RPMI-1640 medium, no L-Glutamine, no calcium | |
| Product Catalog Number | |
| G4537-500ML | |
| Specifications | |
| 500 mL | |
| Product Introduction | |
| Developed by Roswell Park Memorial Institute (RPMI) | |
| Designed for lymphocyte culture, now widely used for various cell cultures including adherent and suspension cells | |
| Contains amino acids, vitamins, and inorganic salts | |
| Protein and growth factor-free | |
| Requires addition of 5-10% serum or serum-free additives based on cell type | |
| Sterile filtered through 0.1 μm membrane, pH 7.0-7.4, contains 11.1 mM D-glucose | |
| Does not contain calcium nitrate, L-Glutamine, sodium pyruvate, HEPES buffer system | |
| Storage and Transportation | |
| Shipped at room temperature; store at 2-8°C | Shelf life: 12 months |
| Precautions | |
| Aseptic handling, avoid contamination | |
| Do not freeze-thaw, do not store completely against refrigerator walls | |
| Note | |
| For research purposes only, not for clinical diagnosis |
| Catalog Number | Component | mg/L | mM |
|---|---|---|---|
| G4537 | Amino Acids | ||
| Glycine | 10 | 0.133 | |
| L-Lysine hydrochloride | 241.86 | 1.149 | |
| L-Asparagine monohydrate | 56.82 | 0.379 | |
| L-Aspartic acid | 20 | 0.15 | |
| L-Cysteine hydrochloride | 65.2 | 0.208 | |
| L-Glutamic acid | 20 | 0.136 | |
| L-Histidine hydrochloride monohydrate | 20.22 | 0.097 | |
| L-Hydroxyproline | 20 | 0.153 | |
| L-Isoleucine | 50 | 0.382 | |
| L-Leucine | 50 | 0.382 | |
| L-Lysine hydrochloride | 40 | 0.219 | |
| L-Methionine | 15 | 0.101 | |
| L-Phenylalanine | 15 | 0.091 | |
| L-Proline | 20 | 0.174 | |
| L-Serine | 30 | 0.286 | |
| L-Threonine | 20 | 0.168 | |
| L-Tryptophan | 5.0 | 0.025 | |
| L-Tyrosine disodium salt dihydrate | 28.83 | 0.111 | |
| L-Valine | 20 | 0.171 | |
| Vitamins | |||
| Biotin | 0.2 | 0.00082 | |
| Choline chloride | 3.0 | 0.02143 | |
| D-Pantothenic acid, calcium salt | 0.25 | 0.00052 | |
| Folic acid | 1.0 | 0.00227 | |
| Niacinamide | 1.0 | 0.00820 | |
| P-Aminobenzoic acid | 1.0 | 0.00730 | |
| Pyridoxine hydrochloride | 1.0 | 0.00485 | |
| Riboflavin | 0.2 | 0.00053 | |
| Thiamine hydrochloride | 1.0 | 0.00297 | |
| Vitamin B12 | 0.005 | 0.00000369 | |
| Myo-Inositol | 35 | 0.19444 | |
| Inorganic Salts | |||
| Magnesium sulfate (anhydrous) | 48.84 | 0.407 | |
| Potassium chloride | 400 | 5.33 | |
| Sodium bicarbonate | 2000 | 23.8 | |
| Sodium chloride | 6000 | 103.4 | |
| Anhydrous sodium phosphate | 800 | 5.63 | |
| Other Components | |||
| D-Glucose | 2000 | 11.11 | |
| Reduced glutathione | 1.0 | 0.003 | |
| Phenol Red | 4.6 | 0.013 |
(Note: Product packaging is being upgraded, subject to physical items.)
