43. RPMI-1640 Medium, GlutaPlus, HEPES, no Sodium Pyruvate

Product Information

Product Description

The RPMI-1640 stands for Roswell Park Memorial Institute, and RPMI-1640 is a cell culture medium developed by the institute. Originally designed specifically for lymphocyte culture, it is now one of the most widely used media for the culture of normal and cancerous cells, particularly in suspension.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.

This product is sterilized by 0.1μm filtration membrane, pH 7.0-7.4, contains 11.1 mM D-glucose, 2.05 mM L-alanyl-L-glutamine,, phenol red indicator, 25 mM HEPES buffer system, no sodium pyruvate.

L-alanyl-L-glutamine, is a cell culture additive that replaces medium moles of L-glutamine in cell culture media. The substance is stable in aqueous solution and does not degrade spontaneously. Instead, it is slowly degraded by a peptidase secreted by cells to release glutamine, which is then absorbed and used by cells.

HEPES is an excellent biological buffering agent with no toxic effect on cells, and the medium supplemented with HEPES can maintain a constant pH range for a long time.

Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

For Research Use Only!

G4532 RPMI-1640 Medium Formula