5. SweScript One-Step RT-PCR Kit, 50 rxns $560

Product Information

Description/Introduction

This product adopts the one-step RT-PCR method, takes RNA as the template, uses gene specific primers, and performs reverse transcription and PCR amplification in the same system. No reagent needs to be added during the reaction, just add RNA template, target gene primers and water supplement system, which can effectively reduce the risk of pollution. This kit contains swescript one step RT mix, 2 × One step PCR master mix, where swescript one step RT mix includes swescript reverse transcriptase and RNase inhibitor. Swescript reverse transcriptase is a mutant obtained through in vitro evolutionary screening. Its RNase H activity is reduced, and it has strong extension ability and thermal stability. two × One step PCR master mix includes DNA polymerase, reaction buffer, dNTPs, mg2+, RT-PCR reaction stabilizer, etc. DNA polymerase has calibration function, and the reaction product is flat end. The DNA polymerase has been modified and optimized to greatly improve the amplification performance and specificity. Under the carefully optimized reaction system and conditions, the amplification product of one step RT-PCR of this kit is up to 8 KB.

Storage and Handling Conditions

Wet ice bag transportation; Stored at -20℃ temperature, valid for 12 months.

Component

Assay Protocol / Procedures

1. Preparation of RT-PCR reaction system

(2) Gently mix and centrifuge;

(3)RT-PCR reaction program setting

a: For high GC or complex templates, the reverse transcription temperature can be raised to 55℃.

b: The annealing temperature is adjusted according to the TM value of the primer. Generally, the annealing temperature is lower than 3-5 ℃ of the TM value.

Note:

1. Please wear disposable gloves during operation to avoid RNase pollution.

2. Be sure to combine swescript one step RT mix and 2 × One step PCR master mix is placed on ice.

3. Swescript one step RT mix contains a high concentration of glycerol, which should be centrifuged briefly before use.

4. When using this kit for reverse transcription reaction, specific reverse transcription primers must be used, and random primer and oligo DT primer cannot be used.

5. It is suggested to use the RNA extracted by the kit as a template to amplify the target gene of more than 5KB.

For Research Use Only!

Ver. No.: V1.0-202207