4. Recombinant HRV 3C Protease (His-tag), 1000 U $488

Product Information

Description

Recombinant HRV 3C Protease is a recombinant expression of Human Rhinovirus type 14 3C Protease with His tag in Escherichia coli. It is a commonly used cysteine protease. It can specifically recognize the octapeptide sequence L-E-V-L-F-Q-G-P (LeU-Glu-Val-Leu-Ph-Gln-Gly-Pro) or the core pentapeptide sequence L-F-Q-G-P (Leu-Ph-Gln-Gly-Pro) at low temperature. The enzyme digestion was performed between Q (Gln) and G-P (Gly-Pro) amino acid residues. The enzyme has strong specificity and high activity, and it still has high activity even at low temperature of 4℃.

Application: It is often used to remove MBP, GST, His or other tag proteins from fusion proteins. The protease itself carries a His tag that binds to the nickel column.

Source: Human Rhinovirus type 14 3C Protease, recombinant expressed in Escherichia coli, containing His tag.

Enzyme activity : It was defined as one unit of enzyme activity required to cleave more than 90% of 100 μg of substrate (protein containing HRV 3C Protease restriction site) within 16 h at 4 ° C and pH 7.5.

Inactivation or inhibition: HRV 3C Protease activity was inhibited by 100 mM ZnCl2, 4 mM AEBSF, and 100 μM Chymostatin.

Purity and concentration: Purity ≥95% by SDS-PAGE, 2 U/μL.

Recombinant HRV 3C Protease storage solution：50 mM Tris-HCl，150 mM NaCl，1 mM EDTA，1 mM DTT，50% (v/v) glycerol，pH 8.0。

10×HRV 3C Buffer：500 mM Tris-HCl，1.5 M NaCl，10 mM EDTA，10 mM DTT，pH 7.5。

Storage and Handling Conditions

Transport with wet ice; Store at -20℃, valid for 12 months.

Component

Assay Protocol

1. Protease digestion system recommended：

2. The reaction system was placed at 4℃ overnight or for 16 h；

3. 10 μL of enzyme digestion product was taken for SDS-PAGE electrophoresis to evaluate the amount of enzyme digestion and reaction conditions.。

Note:

1. Enzyme products should be placed in an ice box or ice bath when used, and stored at -20℃ immediately after use. It is recommended to store them separately；

2. For your safety and health, please wear a lab coat and disposable gloves when operating.

Examples of enzyme digestion applications

Figure 1: Effect of HRV 3C Protease digestion of control substrate protein(A+B fusion protein, which contains HRV 3C Protease restriction site) (The restriction conditions were 30℃, pH 7.5, 0 min, 10 min, 20 min, 30 min, 1 h, 2 h, respectively, corresponding to Lane: 1-6, the mass ratio of added substrate to enzyme in the enzymatic digestion system is 10:1)

For Research Use Only!