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4. Recombinant Anti -GAPDH antibody ( Mouse mAb ) $300 100 μL
$300.00
In Stock
Description
Product Information
Description | Mouse monoclonal to GAPDH |
Protein full name | Glyceraldehyde-3-phosphate dehydrogenase |
Synonyms | GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase |
Immunogen | KLH conjugated synthetic peptide corresponding to Mouse GAPDH |
Isotype | IgG1 |
Purity | Affinity purification |
Subcellular location | Cytoplasm, Nucleus, Membrane |
Predicted MW. | 36kDa |
Observed MW. | 36kDa |
Uniprot ID | P16858 |
Applications
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | heart, lung, liver, kidney, brain |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | breast cancer, esophageal cancer, kidney, testis, spinal cord |
Background
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.
Images
Western blot analysis of GAPDH (GB15002)at dilution of 1: 2000 | |
Western blot analysis of GAPDH (GB15002)at dilution of 1: 2000 | |
Immunohistochemistry of paraffin embedded human breast cancer using GAPDH (GB15002) at dilution of 1: 500 | |
Immunohistochemistry of paraffin embedded human esophageal cancer using GAPDH (GB15002) at dilution of 1: 500 | |
Immunohistochemistry of paraffin embedded mouse kidney using GAPDH (GB15002) at dilution of 1: 500 | |
Immunohistochemistry of paraffin embedded rat kidney using GAPDH (GB15002) at dilution of 1: 500 | |
Immunohistochemistry of paraffin embedded mouse testis using GAPDH (GB15002) at dilution of 1: 500 | |
Immunohistochemistry of paraffin embedded rat spinal cord using GAPDH (GB15002) at dilution of 1: 500 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).