4. Recombinant Anti -GAPDH antibody ( Mouse mAb ) $300 100 μL

SKU: GB15002
$300.00
In Stock
SKU: GB15002 Category:

Description

Product Information

Description Mouse monoclonal to GAPDH
Protein full name Glyceraldehyde-3-phosphate dehydrogenase
Synonyms GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase
Immunogen KLH conjugated synthetic peptide corresponding to Mouse GAPDH
Isotype IgG1
Purity Affinity purification
Subcellular location Cytoplasm, Nucleus, Membrane
Predicted MW. 36kDa
Observed MW. 36kDa
Uniprot ID P16858

Applications

Applications Species Dilution Positive Sample
WB Human, Mouse, Rat 1: 1000-1: 2000 heart, lung, liver, kidney, brain
IHC/IF Human, Mouse, Rat 1: 500-1: 1000 breast cancer, esophageal cancer, kidney, testis, spinal cord

Background

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.


Images

图片 1.png Western blot analysis of GAPDH (GB15002)at dilution of 1: 2000
图片 1.png Western blot analysis of GAPDH (GB15002)at dilution of 1: 2000
图片 2.png Immunohistochemistry of paraffin embedded human breast cancer using GAPDH (GB15002) at dilution of 1: 500
图片 3.png Immunohistochemistry of paraffin embedded human esophageal cancer using GAPDH (GB15002) at dilution of 1: 500
图片 5.png Immunohistochemistry of paraffin embedded mouse kidney using GAPDH (GB15002) at dilution of 1: 500
图片 9.png Immunohistochemistry of paraffin embedded rat kidney using GAPDH (GB15002) at dilution of 1: 500
图片 10.png Immunohistochemistry of paraffin embedded mouse testis using GAPDH (GB15002) at dilution of 1: 500
图片 11.png Immunohistochemistry of paraffin embedded rat spinal cord using GAPDH (GB15002) at dilution of 1: 500

Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).


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