4. Recombinant Anti – beta Actin antibody ( Mouse mAb ); 100 μL $200

SKU: GB11001-100.
$199.00
In Stock
SKU: GB11001-100. Category:

Description

Product Information

Description Mouse monoclonal to beta Actin antibody
Protein full name Actin, cytoplasmic 1
Synonyms ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta CytoskeletalActin, β-Actin
Immunogen KLH conjugated synthetic peptidecorresponding to Mouse beta-Actin
Isotype IgG1
Purity Affinity purification
Subcellular location Cytoplasm
Predicted MW. 42kDa
Observed MW. 45kDa
Uniprot ID P60710

Applications

Applications Species Dilution Positive Sample
WB Human, Mouse, Rat 1: 1000-1: 2000 lung, brain
IHC/IF Human, Mouse, Rat 1: 500-1: 1000 rectum, liver, colon, stomach
ICC/IF Human, Mouse, Rat 1: 300-1: 1000 NIH/3T3, A549, etc.

Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.


Images

Western blot analysis of β-Actin(GB15001)

at dilution of 1: 1000

Western blot analysis of β-Actin(GB15001)

at dilution of 1: 1000

Immunofluorescent analysis of paraformaldehyde-fixed mouse rectum using β-Actin (GB15001) at dilution of 1: 1000
Immunofluorescent analysis of paraformaldehyde-fixed rat small intestine using β-Actin (GB15001) at dilution of 1: 1000
Immunofluorescent analysis of paraformaldehyde-fixed human colon using β-Actin (GB15001) at dilution of 1: 1000
Immunocytochemistry analysis of 4% paraformaldehyde fixed NIH/3T3 cells using β-Actin (GB15001) at dilution of 1: 1000
Immunocytochemistry analysis of 4% paraformaldehyde fixed A549 cells using β-Actin (GB15001) at dilution of 1: 1000

Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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