4. Ice-Bath Free Fast Transfer Buffer (Powder),1 L (Powder)*15 pouches, $120

Description

Product Information

Product Description

This product is a safe and harmless buffer for rapid wet membrane transfer in Western Blot, with efficient and rapid transfer of proteins to blotting membranes in 15-30 min without the use of methanol.

Safe and non-toxic: This product does not contain toxic and harmful components, and does not need to use highly toxic reagents such as methanol.

The transfer effect of the transfer buffer is the same or better than that of the traditional transfer buffer.

Rapid membrane transfer without ice bath: With rapid membrane transfer buffer, the transfer of proteins from polyacrylamide gels to blotting membranes such as PVDF or nitrocellulose (NC) membranes can be carried out at a constant flow membrane of 400 mA for 15-30 min, with lower heat production than with conventional transfer buffer.

Storage and Shipping Conditions

Ship at room temperature; Store in a dry place, valid for 24 months.

Product Component

Assay Protocol

1. Dissolve each package of ice-bath free fast film transfer buffer powder in 800 mL of pure water, add 200 mL of absolute ethanol to dissolve and mix thoroughly(multiply as required);

2. Complete the sandwich structure required for transfer printing, from the negative electrode (black side) to the positive electrode (red side) in the order of sponge, filter paper, gel, blotting film, filter paper, sponge, and then transfer to the transfer tank, set a constant current of 400 mA, film transfer time of 15-30 min (this condition is for 1.0mm thick gel, if the gel thickness is 0.75mm , appropriately reduce the transfer time; If the gel thickness is 1.5 mm, extend the transfer time appropriately) to complete the protein transfer.

Note

1. This product is suitable for the membrane transfer of PAGE gel of Tris-Gly, Bis-Tris and other buffer systems.

2. PVDF membrane should be infiltrated with absolute ethanol for about 30 s before use.

3. The transfer current is recommended to be set at a constant current of 400 mA. Generally, 5-15 min for proteins below 20 kDa, 15-25 min for 20-150 kDa and 25-35 min for proteins above 150 kDa.

4. Ice bath is not required for film transfer within 30 min. Ice bath is recommended for film transfer over 30 min.

5. Due to the different brand and model of the power supply used for the film, the maximum current varies greatly and the power varies greatly on the other; some power supplies set at a constant current of 400 mA will exceed the maximum power and overload protection will occur, so it is necessary to adjust the current downwards or use a power supply with more power.

6. The temperature of the transfer buffer, gel parameters (such as thickness, acrylamide concentration, ion concentration), filter paper thickness, volume of transfer buffer and times of use of transfer buffer may affect the current and voltage; In case of overload, please reduce the current and prolong the film transfer time.

7. The normal color of this product is colorless and transparent. If light brown or yellowish brown appears, it should be discarded promptly.

8. For your safety and health, please wear safety glasses, gloves, or protective clothing.

For Research Use Only!