33. α-MEM, GlutaPlus, no Nucleosides

SKU: G4555-500ML
$40.00
In Stock
SKU: G4555-500ML Categories: ,

Description

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Product Information

Product Name Cat. No. Spec.
α-MEM, GlutaPlus, no nucleosides G4555-500ML 500 mL

 


Product Description/Introduction

α-MEM (α Minimum Essential Medium) is a modified medium from MEM. Compared with MEM , α-MEM has added sodium pyruvate, zinc sulfate, vitamin B12, biotin, ascorbic acid and other components, and is widely used for the culture of various mammalian suspension and adherent cells. α-MEM is divided into the types of nucleoside-containing and non-nucleoside-containing. The nucleoside-free and deoxynucleoside-free α-MEM is commonly used as a screening medium for DG44 and other DHFR-deficient cells.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add serum or serum-free additives depending on the cell type.

This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains L-Alanyl-L-Glutamine, sodium pyruvate, phenol red indicator, without HEPES and nucleosides. Please check the official website for the specific recipe.

 


Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

 

For Research Use Only!

G4555 αMEM Formula

Components Concentration Components Concentration
mg/L mM mg/L mM
Amino acids Inorganic salts
Glycine 50.0 0.6667 Calcium Chloride (CaCl2)(anhyd.) 200 1.801802
L-Alanine 25.0 0.2809 Magnesium Sulfate (MgSO4) (anhyd.) 97.67 0.813917
L-Arginine hydrochloride 105.0 0.4976 Potassium Chloride (KCl) 400 5.333
L-Asparagine-H2O 50.0 0.3333 Sodium Bicarbonate (NaHCO3) 2200 26.2
L-Aspartic Acid 30.0 0.2256 Sodium Chloride (NaCl) 6800 117.2
L-Cystine 2HCl 31.0 0.0990 Sodium Phosphate monobasic (NaH2PO4) (anhyd.) 121.7 1.014
L-Cysteine hydrochloride-H2O 100.0 0.5682 Other components
L-Glutamic Acid 75.0 0.5102 D-Glucose (Dextrose) 1000 5.6
L-Alanyl-L-Glutamine 434.4 2.0 Lipoic Acid 0.20 0.0009709
L-Histidine hydrochloride-H2O 42 0.20 Phenol Red 9.40 0.026567
L-Isoleucine 52.4 0.40 Sodium pyruvate 110 1.0
L-Leucine 52.0 0.3969
L-Lysine hydrochloride 73.0 0.3989
L-Methionine 15.0 0.1007
L-Phenylalanine 32.0 0.1939
L-Proline 40.0 0.3478
L-Serine 25.0 0.2381
L-Threonine 48.0 0.4034
L-Tryptophan 10.0 0.0490
L-Tyrosine Disodium Salt 52.0 0.2311
L-Valine 46.0 0.3932
Vitamins
Ascorbic Acid 50.0 0.2841
Biotin 0.10 0.000410
Choline chloride 1.0 0.007143
D-Calcium pantothenate 1.0 0.002096
Folic Acid 1.0 0.002268
Niacinamide 1.0 0.008197
Pyridoxine hydrochloride 1.0 0.004902
Riboflavin 0.10 0.000266
Thiamine hydrochloride 1.0 0.002967
Vitamin B12 1.36 0.001004
i-Inositol 2.0 0.011111

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