3. Plant Tissue Bating Liquor; 500mlx2 $ 83.9

Product Information

Description

When making sections, plant tissues such as roots and stems with a high degree of lignification cannot be sliced directly because the tissues are hard and brittle, and tissue sections need to be softened before tissue sections can be obtained. This product is used for the softening of lignified plant tissues, and the xylem structure is destroyed by alkaline solution to achieve softening, and the softened plant tissues can be easily prepared into tissue sections, and the tissue structure is intact. The main active ingredient of this product is ethylenediamine.

Storage and Handling Conditions

Store and transport at room temperature; valid for 12 months.

Assay Protocol

The plant tissue passed FAA fixed time of about 48 h immediately after in vitro. Cut the fixed tissue into a suitable size, generally not more than 0.5 cm thick is appropriate, put into the softening liquid so that the tissue is completely immersed in the softening liquid, begin to soften, tissue softening period every 3-5 days to replace fresh softening liquid. Softening is completed when the tissue is flexible enough to be touched by the hand and can be easily cut with a surgical blade. The softening time for an average-sized tissue is about a week. For tissues with higher lignification, the softening time is longer.

Note:

1. Please use the product in a well-ventilated place with proper protection to avoid inhalation.

2. This product can achieve plant softening by destroying xylem structure, which has an impact on subsequent saffron staining. Use with caution.

3. If subsequent tissue sections need to be stained with saffron fast green to observe xylem structure, it is recommended to make hard tissue sections (GP1017, GP1018, GP1020) embedded in plastic.

4. Please wear laboratory coat and disposable gloves during operation.

For Research Use Only!