No products in the cart.
2. HAT Complete Medium, 100 ml $200
$200.00
In Stock
Description
Please click here to see the video. thanks
and another video to see the product quality.
Product Information
Product Name: HAT Complete Medium
Product Code: G4106-100ML
Specification: 100 mL
Product Overview: This product is a complete medium containing HAT (Hypoxanthine, Aminopterin, Thymidine) for the selection and primary culture of hybridoma cells in the process of monoclonal antibody preparation from rat, mouse, and hamster species. The specific components are as follows:
Components:
| Component | Content |
|---|---|
| RPMI-1640 Basal Medium | 80% (v/v) |
| Fetal Bovine Serum | 15% (v/v) |
| Penicillin | 1000000 U/mL |
| Streptomycin | 0.1 μg/mL |
| Hypoxanthine | 0.0001 mol |
| Thymidine | 0.000016 mol |
| Aminopterin | 0.0000004 mol |
Storage and Transportation: Transport at 4°C, store in a light-protected manner at -20°C. Shelf life is 18 months.
Composition:
| Component Number | Component |
|---|---|
| G4106-100ML | HAT Complete Medium (1 bottle) |
| Instruction Manual | 1 copy |
Usage Instructions:
- This product can be used after being shaken well when completely melted at room temperature or 37°C.
- Centrifuge and collect the fused hybridoma cells, discard the supernatant containing residual PEG, and resuspend the cells with this product to the appropriate density (typically, 5-10 plates for mouse or hamster spleen fusion, and 20-30 plates for rat fusion). Then evenly distribute the cells into a 96-well cell culture plate (about 200 μL per well). Hybridoma cell colonies can be observed under a microscope in 4-7 days.
Notes:
- This product is a sterile working solution. Shake well after completely melting at room temperature or 37°C. A small amount of insoluble precipitate after melting is normal.
- One batch of this product can cover 5 plates of a 96-well plate. It is recommended to use the remaining portion after thawing for a single experiment and promptly return it to -20°C storage. Avoid repeated opening and freezing.
- This product is intended for the selection of hybridoma cells after cell fusion and the preparation of mouse monoclonal antibodies. It cannot be used directly for culturing HGPRT-deficient cells such as SP2/0.
- This product has some level of toxicity. Gloves and masks should be worn during operation.
This product is for research purposes only and not for clinical diagnosis.
(Note: Product packaging may be upgraded; refer to the actual product for details.)
