4. Permeabilization Solution, 100 ml $98

Product Information

Description

A membrane breaker is used to treat the cell membrane before staining for intracellular cytokines or proteins. It can create holes in the cell membrane so that dyes or antibodies can enter the cell. Commonly used membrane breaking methods mainly consist of organic solvents, such as methanol and acetone, etc. These solvents mainly achieve fixation and membrane breaking by dissolving membrane lipids and condensing membrane proteins. Descaling agents, such as Triton X-100 and Saponin, can also be used to achieve membrane breaking through non-specific membrane lysis.

The active component of the solution was 0.5% Triton X-100, which was used to permeate the cell membrane before staining or immunolabelling.

Storage and Handling Conditions

Wet ice transportation; Store at 4℃, valid for 12 months.

Assay Protocol

1. For tissue sections: After fixation and washing, add broken membrane solution to cover the tissue, incubate at room temperature for 20-30 min, and then place the glass slides in PBS (G4202) and shake them on a decolorization shaker for 3 times, 5 min each time. You can proceed to the next step.

2. For the cells in the well plate: after fixing and washing, drop the membrane breaking solution to cover the cells, incubate at room temperature for 10-20 min, and then wash with PBS for 3 times, 5 min each time.

Note:

1. For cell samples, if Triton X-100 less than 0.5% is required, the product can be diluted with PBS buffer before use.

2. For the samples that are difficult to penetrate, the processing time can be extended as appropriate.

3. Wear a lab coat and disposable gloves during operation.

For Research Use Only!