3. 10× Tris-Glycine Transfer Buffer, 1L (10X) $ 65

Product Information

Product Description

10× Tris-Glycine Transfer buffer is suitable for wet or semi-dry transfer of Western-Blot experiments.

This product is a 10-fold concentrate consisting of 200 mM Tris, 1.92 M glycine, without methanol or anhydrous ethanol. Mix 100 mL of this product with 200 mL of anhydrous ethanol or methanol, then add deionized water or distilled water to 1 L to obtain 1x transfer solution of 20 mM Tris, 192 mM glycine, 20% anhydrous ethanol, pH 8.2-8.6 at 25°C.

Storage and Shipping Conditions

Ship and store at room temperature, valid for 12 months.

Assay Protocol

1. For conventional small to medium molecular weight proteins (25-70 kDa), the recommended conditions for transfer are 300 mA for about 30 min.

2. For conventional large molecular weight proteins, the transfer time can be extended as appropriate, with a recommended transfer time of 300 mA for 45-60 min.

3. For small molecular weight proteins below 20 kDa, it is recommended to use a smaller pore size of 0.22 μm PVDF membrane with 200 mA for 20-25 min or 300 mA for 15-20 min. The methanol content (which can be replaced by ethanol) can also be increased to 25% to enhance the fixation of small molecular proteins in the gel.

4. For proteins with particularly large molecular weight, it is recommended to extend the transfer time to 300 mA for 60-90 min or longer, add an appropriate amount of SDS to the transfer solution to the final concentration of about 0.5‰ to enhance the release of proteins from the gel. At the same time, due to the long transfer time, attention should be paid to the effect of the cooling module, a new cooling module can be replaced halfway if necessary.

Note

1.  Use quickly after dissolving.

2.  For your safety and health, please wear safety glasses, gloves, or protective clothing.

For Research Use Only!