29. MEM, NEAA, GlutaPlus, HEPES

Product Information

Product Description

Minimum Essential Medium (MEM), which is also known as Low Limited Eagle Medium, is one of the most commonly used basic media in animal cell culture. MEM contains only 12 kinds of essential amino acids, 8 kinds of vitamins and basic inorganic salts, and has simple nutritional composition. It is mainly used for culture of adherent cells, and can also be used for culture of other types of cells culture with a modified formulation.

MEM containing NEAA (non-essential amino acids) is supplemented with seven kinds of NEAA, including L-alanine, L-glutamic acid, L-asparagine, L-aspartate, L-proline, L-serine and glycine, which can reduce the side effects of non-essential amino acid synthesis by cells during cell culture and effectively promote cell proliferation and metabolism.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, and does not contain protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.

This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains 5.5 mM D-glucose and non-essential amino acids, 2.0 mM L-Alanyl-L-Glutamine, phenol red indicator, 25mM HEPES buffer system, no sodium pyruvate.

HEPES is a commonly used biological buffering agent, which can keep the medium at a constant pH range for a long time and effectively prevent the adverse effects of large pH fluctuations on cell growth.

Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

For Research Use Only!

G4551 MEM, with NEAA, HEPES