Description
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Product Information
Product Name | Cat. No. | Spec. |
MEM, NEAA, no L-Glutamine, no HEPES | G4550-500ML | 500 mL |
Product Description/Introduction
MEM (Minimum Essential Medium), also known as minimum basic medium, low limit Eagle medium, is one of the most commonly used basic mediums in animal cell culture. MEM contains only 12 amino acids, 8 vitamins and basic inorganic salts, with a simple nutrient composition, is mainly used for the culture of walled cells, but can also be used for other types of cell culture after the formulation is revised.
MEM which contains NEAA (non-essential amino acids) is based on MEM with the addition of 7 NEAA, including L-Alanine, L-Glutamic acid, L-Asparagine, L-Aspartic acid, L-Proline, L-Serine and Glycine, to reduce the side effects of cells’ own synthesis of non-essential amino acids during cell culture and effectively promote cell proliferation and metabolism.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add serum or serum-free additives depending on the cell type.
This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 5.5 mM D-glucose, phenol red indicator, without L-glutamine, sodium pyruvate and HEPES.
Storage and Shipping Conditions
Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. For best results, do not freeze and thaw repeatedly.
3. For your safety and health, please wear safety glasses, gloves or protective clothing.
For Research Use Only!
G4550 MEM, with NEAA
Components | Concentration | Components | Concentration | |||
mg/L | mM | mg/L | mM | |||
Amino acids | Inorganic salts | |||||
L-Arginine hydrochloride | 126.0 | 0.5972 | Calcium Chloride (CaCl2)(anhyd.) | 200 | 1.802 | |
L-Cystine 2HCl | 31.0 | 0.09904 | Magnesium Sulfate (MgSO4) (anhyd.) | 200 | 0.813 | |
L-Glutamine | – | – | Potassium Chloride (KCl) | 400 | 5.333 | |
L-Histidine hydrochloride-H2O | 42.0 | 0.2 | Sodium Bicarbonate (NaHCO3) | 2200 | 26.19 | |
L-Isoleucine | 52.0 | 0.3969 | Sodium Chloride (NaCl) | 6800 | 117.2 | |
L-Leucine | 52.0 | 0.3969 | Sodium Phosphate monobasic (NaH2PO4) (anhyd.) | 122 | 1.014 | |
L-Lysine hydrochloride | 73.0 | 0.3989 | Other components | |||
L-Methionine | 15.0 | 0.1007 | D-Glucose (Dextrose) | 1000 | 5.556 | |
L-Phenylalanine | 32.0 | 0.1939 | Phenol Red | 9.4 | 0.02657 | |
L-Threonine | 48.0 | 0.4034 | HEPES | – | – | |
L-Tryptophan | 10.0 | 0.04902 | ||||
L-Tyrosine disodium salt dihydrate | 52.0 | 0.1992 | ||||
L-Valine | 46.0 | 0.3932 | ||||
Glycine | 8.0 | 0.1067 | ||||
L-Alanine | 9.0 | 0.1011 | ||||
L-Asparagine monohydrate | 14.8 | 0.09848 | ||||
L-Asparagic acid | 13.0 | 0.09774 | ||||
L-Aminoglutaric acid | 15.0 | 0.1020 | ||||
L-Proline | 12.0 | 0.1043 | ||||
L-Serine | 11.0 | 0.1048 | ||||
Vitamins | ||||||
Choline chloride | 1.0 | 0.007143 | ||||
D-Calcium pantothenate | 1.0 | 0.002096 | ||||
Folic Acid | 1.0 | 0.002268 | ||||
Niacinamide | 1.0 | 0.008197 | ||||
Pyridoxine hydrochloride | 1.0 | 0.004902 | ||||
Riboflavin | 0.1 | 0.0002660 | ||||
Thiamine hydrochloride | 1.0 | 0.002967 | ||||
i-Inositol | 2.0 | 0.01111 |