27. DMEM, Low Glucose, HEPES, no L-Glutamine, no Sodium Pyruvate

SKU: G4521-500ML
$40.00
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SKU: G4521-500ML Categories: ,

Description

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Product Information

Product Name Cat.No. Spec.
DMEM,Low Glucose HEPES,no L-Glutamine,no sodium pyruvate G4521-500ML 500 mL

 


Introduction 

DMEM, namely Dulbecco’s Modified Eagle Medium, is modified on the basis of MEM medium and can be divided into low glucose and high glucose according to different glucose content. DMEM low glucose medium is suitable for the culture of slow metabolic and dependent adherent cells.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Cells were cultured with 5-10% serum or serum-free additives, depending on the cell type.

This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, containing 5.5 mM D-glucose, no L-glutamine and sodium pyruvate, added phenol red indicator, containing 25 mM HEPES buffer system.

HEPES is an excellent biological buffering agent with no toxic effect on cells. The medium supplemented with HEPES can maintain a constant pH range for a long time.


Storage and Handling Conditions

Transport at room temperature; Store at 2-8℃, valid for 12 months.


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation during use to avoid contamination;;

2. In order to maintain the best use effect, do not freeze and thaw repeatedly.

 

For Research Use Only!

DMEM(Low Glucose) Medium Formula

Components Concentration Components Concentration 
mg/L mM mg/L mM
Amino acids Inorganic salts
Glycine 30 0.4 Calcium Chloride (CaCl2)(anhyd.) 200 1.80
L-Arginine hydrochloride 84 0.4 Ferric nitrate nonahydrate ( Fe(NO3)3•9H2O) 0.1 0.0002
L-Cystine dihydrochloride 62.6 0.2 Magnesium Sulfate (MgSO4) (anhyd.) 97.67 0.80
L-Histidine hydrochloride-H2O 42 0.2 Potassium Chloride (KCl) 400 5.36
L-Isoleucine 105 0.8 Sodium Bicarbonate (NaHCO3) 3700 44.04
L-Leucine 105 0.8 Sodium Chloride (NaCl) 6400 109.50
L-Lysine hydrochloride 146 0.8 Sodium Phosphate monobasic (NaH2PO4) (anhyd.) 108.7 0.906
L-Methionine 30 0.2 Other components
L-Phenylalanine 66 0.4 D-Glucose (Dextrose) 1000 5.55
L-Serine 42 0.4 Sodium pyruvate
L-Threonine 95 0.8 HEPES 5958 25
L-Tryptophan 16 0.08 Phenol Red 14.95 0.0422
L-Tyrosine disodium salt dihydrate 103.79 0.4
L-Valine 94 0.8
L-Glutamine
L-Alanyl-L-Glutamine
Vitamins
Choline chloride 4.0 0.0286
D-Calcium pantothenate 4.0 0.0084
Folic Acid 4.0 0.0091
Niacinamide 4.0 0.0328
Pyridoxine hydrochloride 4.0 0.0195
Riboflavin 0.4 0.0011
Thiamine hydrochloride 4.0 0.0119
i-Inositol 7.2 0.0389

 

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