23. DMEM, High Glucose, GlutaPlus, HEPES, Sodium Pyruvate

SKU: G4515-500ML
$40.00
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Description

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Product Information

Product Name Cat. No. Spec.
DMEM, High Glucose, GlutaPlus, HEPES, Sodium Pyruvate G4515-500ML 500 mL

 


Product Description

Dulbecco’s Modified Eagle Medium (DMEM), is a modified medium based on MEM, is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add 5-10% serum or serum-free additives depending on the cell type.

This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains 25 mM D-glucose, 4.0mM L-alanyl-L-glutamine, 1.0mM sodium pyruvate, phenol red indicator and 25 mM HEPES buffer system.

HEPES is an excellent biological buffer with no toxic effect on cells. The medium supplemented with HEPES can maintain a constant pH range for a long time.

 


Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

 

DMEM/High Glucose, without L-Glutamine

Components Concentration Components Concentration 
mg/L mM mg/L mM
Amino acids Inorganic salts
Glycine 30 0.4 Calcium Chloride (CaCl2) (anhyd.) 200 1.80
L-Arginine hydrochloride 84 0.4 Ferric nitrate nonahydrate ( Fe(NO3)3•9H2O) 0.1 0.0002
L-Cystine dihydrochloride 62.6 0.2 Magnesium Sulfate (MgSO4) (anhyd.) 97.67 0.80
L-Histidine hydrochloride-H2O 42 0.2 Potassium Chloride (KCl) 400 5.36
L-Isoleucine 105 0.8 Sodium Bicarbonate (NaHCO3) 3700 44.04
L-Leucine 105 0.80 Sodium Chloride (NaCl) 6400 109.50
L-Lysine hydrochloride 146 0.8 Sodium Phosphate monobasic (NaH2PO4) (anhyd.) 108.7 0.906
L-Methionine 30 0.2 Other components
L-Phenylalanine 66 0.4 D-Glucose (Dextrose) 4500 24.98
L-Serine 42 0.4 Sodium pyruvate 110 1.0
L-Threonine 95 0.8 HEPES
L-Tryptophan 16 0.08 Phenol Red 14.95 0.0422
L-Tyrosine disodium salt dihydrate 103.79 0.4
L-Valine 94 0.8
L-Glutamine
L-Alanyl-L-Glutamine
Vitamins
Choline chloride 4.0 0.0286
D-Calcium pantothenate 4.0 0.0084
Folic Acid 4.0 0.0091
Niacinamide 4.0 0.0328
Pyridoxine hydrochloride 4.0 0.0195
Riboflavin 0.4 0.0011
Thiamine hydrochloride 4.0 0.0119
i-Inositol 7.2 0.0389
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