23. DMEM, High Glucose, GlutaPlus, HEPES, Sodium Pyruvate
Description
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Product Information
Product Name | Cat. No. | Spec. |
DMEM, High Glucose, GlutaPlus, HEPES, Sodium Pyruvate | G4515-500ML | 500 mL |
Product Description
Dulbecco’s Modified Eagle Medium (DMEM), is a modified medium based on MEM, is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add 5-10% serum or serum-free additives depending on the cell type.
This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains 25 mM D-glucose, 4.0mM L-alanyl-L-glutamine, 1.0mM sodium pyruvate, phenol red indicator and 25 mM HEPES buffer system.
HEPES is an excellent biological buffer with no toxic effect on cells. The medium supplemented with HEPES can maintain a constant pH range for a long time.
Storage and Shipping Conditions
Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. For best results, do not freeze and thaw repeatedly.
3. For your safety and health, please wear safety glasses, gloves and protective clothing.
DMEM/High Glucose, without L-Glutamine
Components | Concentration | Components | Concentration | |||
mg/L | mM | mg/L | mM | |||
Amino acids | Inorganic salts | |||||
Glycine | 30 | 0.4 | Calcium Chloride (CaCl2) (anhyd.) | 200 | 1.80 | |
L-Arginine hydrochloride | 84 | 0.4 | Ferric nitrate nonahydrate ( Fe(NO3)3•9H2O) | 0.1 | 0.0002 | |
L-Cystine dihydrochloride | 62.6 | 0.2 | Magnesium Sulfate (MgSO4) (anhyd.) | 97.67 | 0.80 | |
L-Histidine hydrochloride-H2O | 42 | 0.2 | Potassium Chloride (KCl) | 400 | 5.36 | |
L-Isoleucine | 105 | 0.8 | Sodium Bicarbonate (NaHCO3) | 3700 | 44.04 | |
L-Leucine | 105 | 0.80 | Sodium Chloride (NaCl) | 6400 | 109.50 | |
L-Lysine hydrochloride | 146 | 0.8 | Sodium Phosphate monobasic (NaH2PO4) (anhyd.) | 108.7 | 0.906 | |
L-Methionine | 30 | 0.2 | Other components | |||
L-Phenylalanine | 66 | 0.4 | D-Glucose (Dextrose) | 4500 | 24.98 | |
L-Serine | 42 | 0.4 | Sodium pyruvate | 110 | 1.0 | |
L-Threonine | 95 | 0.8 | HEPES | – | – | |
L-Tryptophan | 16 | 0.08 | Phenol Red | 14.95 | 0.0422 | |
L-Tyrosine disodium salt dihydrate | 103.79 | 0.4 | ||||
L-Valine | 94 | 0.8 | ||||
L-Glutamine | – | – | ||||
L-Alanyl-L-Glutamine | – | – | ||||
Vitamins | ||||||
Choline chloride | 4.0 | 0.0286 | ||||
D-Calcium pantothenate | 4.0 | 0.0084 | ||||
Folic Acid | 4.0 | 0.0091 | ||||
Niacinamide | 4.0 | 0.0328 | ||||
Pyridoxine hydrochloride | 4.0 | 0.0195 | ||||
Riboflavin | 0.4 | 0.0011 | ||||
Thiamine hydrochloride | 4.0 | 0.0119 | ||||
i-Inositol | 7.2 | 0.0389 |
Cat.No.
|
Product Name
|
Spec.
|
Operation
|
---|
G4511-500ML
|
DMEM, High Glucose, GlutaPlus, Sodium Pyruvate, no HEPES
|
500 mL
|
|
G4511DLEU-500ML
|
DMEM, High Glucose, GlutaPlus, no Leucine
|
500 mL
|
|
G4512-500ML
|
DMEM, High Glucose, GlutaPlus, HEPES, no Sodium Pyruvate
|
500 mL
|
|
G4514-500ML
|
DMEM,High Glucose no L-Glutamine,no HEPES,no sodium pyruvate
|
500 mL
|
|
G4515-500ML
|
DMEM, High Glucose, GlutaPlus, HEPES, Sodium Pyruvate
|
500 mL
|
|
G4516-500ML
|
DMEM, High Glucose HEPES, no L-Glutamine, no Sodium Pyruvate
|
500 mL
|
|
G4517-500ML
|
DMEM, High Glucose, HEPES, Sodium Pyruvate, no L-Glutamine
|
500 mL
|
|
G4523-500ML
|
DMEM, High Glucose, GlutaPlus, no HEPES, no Sodium Pyruvate
|
500mL
|
|
G4524-500ML
|
DMEM, High Glucose, GlutaPlus, 1.5 g/L Sodium Bicarbonate
|
500 mL
|
|
G4525-500ML
|
DMEM, High Glucose, GlutaPlus, no Phenol Red
|
500 mL
|
Cat.No.
|
Product Name
|
Spec.
|
Operation
|
---|
G4511-500ML
|
DMEM, High Glucose, GlutaPlus, Sodium Pyruvate, no HEPES
|
500 mL
|
|
G4511DLEU-500ML
|
DMEM, High Glucose, GlutaPlus, no Leucine
|
500 mL
|
|
G4512-500ML
|
DMEM, High Glucose, GlutaPlus, HEPES, no Sodium Pyruvate
|
500 mL
|
|
G4514-500ML
|
DMEM,High Glucose no L-Glutamine,no HEPES,no sodium pyruvate
|
500 mL
|
|
G4515-500ML
|
DMEM, High Glucose, GlutaPlus, HEPES, Sodium Pyruvate
|
500 mL
|
|
G4516-500ML
|
DMEM, High Glucose HEPES, no L-Glutamine, no Sodium Pyruvate
|
500 mL
|
|
G4517-500ML
|
DMEM, High Glucose, HEPES, Sodium Pyruvate, no L-Glutamine
|
500 mL
|
|
G4523-500ML
|
DMEM, High Glucose, GlutaPlus, no HEPES, no Sodium Pyruvate
|
500mL
|
|
G4524-500ML
|
DMEM, High Glucose, GlutaPlus, 1.5 g/L Sodium Bicarbonate
|
500 mL
|
|
G4525-500ML
|
DMEM, High Glucose, GlutaPlus, no Phenol Red
|
500 mL
|