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22. DMEM,High Glucose no L-Glutamine,no HEPES,no sodium pyruvate
$40.00
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Description
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Product Content
| Product Name | Cat. No. | Spec. |
| DMEM,High Glucose no L-Glutamine,no HEPES,no sodium pyruvate | G4514-500ML | 500 mL |
Product Description
DMEM (Dulbecco’s Modified Eagle Medium) is one of the most commonly used basal medium for supporting the growth of many different mammalian cells. DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium. Servicebio DMEM media preparations are available in high or low glucose or no glucose formulations, as well as with or without L-glutamine and sodium pyruvate. Servicebio DMEM media include phenol red as a pH indicator. All available DMEM media products contain a sodium bicarbonate buffer with or without HEPES to maintain optimal culture pH.
DMEM is sutiable for primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as Hela, 293, Cos-7 and PC-12. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
All raw material components used for Servicebio cell culture media products are screened through using strict quality control testing. Servicebio cell culture media are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of cell culture media are subjected to sterility test, pH, osmolality, endotoxin and cell culture test.
The major character of this DMEM is as follows:
l With 25 mM D-Glucose, phenol red.
l Without HEPES, L-glutamine, sodium pyruvate.
The complete formulation is available.
Storage
Storage conditions: 2℃ to 8℃, protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
For Research Use Only!
DMEM/High Glucose, without L-Glutamine
| Components | Concentration | Components | Concentration | |||
| mg/L | mM | mg/L | mM | |||
| Amino acids | Inorganic salts | |||||
| Glycine | 30 | 0.4 | Calcium Chloride (CaCl2) (anhyd.) | 200 | 1.80 | |
| L-Arginine hydrochloride | 84 | 0.4 | Ferric nitrate nonahydrate ( Fe(NO3)3•9H2O) | 0.1 | 0.0002 | |
| L-Cystine dihydrochloride | 62.6 | 0.2 | Magnesium Sulfate (MgSO4) (anhyd.) | 97.67 | 0.80 | |
| L-Histidine hydrochloride-H2O | 42 | 0.2 | Potassium Chloride (KCl) | 400 | 5.36 | |
| L-Isoleucine | 105 | 0.8 | Sodium Bicarbonate (NaHCO3) | 3700 | 44.04 | |
| L-Leucine | 105 | 0.80 | Sodium Chloride (NaCl) | 6400 | 109.50 | |
| L-Lysine hydrochloride | 146 | 0.8 | Sodium Phosphate monobasic (NaH2PO4) (anhyd.) | 108.7 | 0.906 | |
| L-Methionine | 30 | 0.2 | Other components | |||
| L-Phenylalanine | 66 | 0.4 | D-Glucose (Dextrose) | 4500 | 24.98 | |
| L-Serine | 42 | 0.4 | Sodium pyruvate | 110 | 1.0 | |
| L-Threonine | 95 | 0.8 | HEPES | – | – | |
| L-Tryptophan | 16 | 0.08 | Phenol Red | 14.95 | 0.0422 | |
| L-Tyrosine disodium salt dihydrate | 103.79 | 0.4 | ||||
| L-Valine | 94 | 0.8 | ||||
| L-Glutamine | – | – | ||||
| L-Alanyl-L-Glutamine | – | – | ||||
| Vitamins | ||||||
| Choline chloride | 4.0 | 0.0286 | ||||
| D-Calcium pantothenate | 4.0 | 0.0084 | ||||
| Folic Acid | 4.0 | 0.0091 | ||||
| Niacinamide | 4.0 | 0.0328 | ||||
| Pyridoxine hydrochloride | 4.0 | 0.0195 | ||||
| Riboflavin | 0.4 | 0.0011 | ||||
| Thiamine hydrochloride | 4.0 | 0.0119 | ||||
| i-Inositol | 7.2 | 0.0389 | ||||
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Product Name
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Spec.
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Operation
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|---|
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G4511-500ML
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DMEM, High Glucose, GlutaPlus, Sodium Pyruvate, no HEPES
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500 mL
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500 mL
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G4515-500ML
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G4516-500ML
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G4517-500ML
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DMEM, High Glucose, HEPES, Sodium Pyruvate, no L-Glutamine
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G4523-500ML
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DMEM, High Glucose, GlutaPlus, no HEPES, no Sodium Pyruvate
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|
G4524-500ML
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DMEM, High Glucose, GlutaPlus, 1.5 g/L Sodium Bicarbonate
|
500 mL
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|
|
G4525-500ML
|
DMEM, High Glucose, GlutaPlus, no Phenol Red
|
500 mL
|
|
Cat.No.
|
Product Name
|
Spec.
|
Operation
|
|---|
|
G4511-500ML
|
DMEM, High Glucose, GlutaPlus, Sodium Pyruvate, no HEPES
|
500 mL
|
|
|
G4511DLEU-500ML
|
DMEM, High Glucose, GlutaPlus, no Leucine
|
500 mL
|
|
|
G4512-500ML
|
DMEM, High Glucose, GlutaPlus, HEPES, no Sodium Pyruvate
|
500 mL
|
|
|
G4514-500ML
|
DMEM,High Glucose no L-Glutamine,no HEPES,no sodium pyruvate
|
500 mL
|
|
|
G4515-500ML
|
DMEM, High Glucose, GlutaPlus, HEPES, Sodium Pyruvate
|
500 mL
|
|
|
G4516-500ML
|
DMEM, High Glucose HEPES, no L-Glutamine, no Sodium Pyruvate
|
500 mL
|
|
|
G4517-500ML
|
DMEM, High Glucose, HEPES, Sodium Pyruvate, no L-Glutamine
|
500 mL
|
|
|
G4523-500ML
|
DMEM, High Glucose, GlutaPlus, no HEPES, no Sodium Pyruvate
|
500mL
|
|
|
G4524-500ML
|
DMEM, High Glucose, GlutaPlus, 1.5 g/L Sodium Bicarbonate
|
500 mL
|
|
|
G4525-500ML
|
DMEM, High Glucose, GlutaPlus, no Phenol Red
|
500 mL
|
