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2. Phosphoprotease inhibitors (set) ( 999 T),1 mL×2 (1ul per test), (for cell and tissues) $248
$248.00
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Description
Product IntroductionRelated Products
Product Information
| Product Name | Cat.No. | Spec. |
| Phosphoprotease Inhibitor | G2007-1ML | 1 mL |
Description
Under stable conditions, the synthesis and breakdown of proteins in cells are in dynamic equilibrium. However, when extracting proteins from cells and tissues under in vitro conditions, cells contain many endogenous proteases that can degrade proteins, such as phosphatases and serine proteases. These ubiquitous endogenous proteases can lead to the decomposition of target proteins, thus affecting the experimental results. To avoid protein degradation, protease inhibitors are usually added during protein extraction. Phosphatase inhibitors are also essential in the study of phosphorylated proteins.
The main components of this product are NaF and Na3VO4, which can inhibit tyrosine phosphatase, acid phosphatase and alkaline phosphatase.
Storage and Handling Conditions
Transport with dry ice；Store at -20℃, valid for 12 months.
Component
| Component Number | Component | G2007-1ML |
| G2007-1 | Phosphoprotease inhibitor A solution (1 M NaF) | 1 mL |
| G2007-2 | Phosphoprotease inhibitor B solution (100 mM Na3VO4) | 1 mL |
| Product Manual | ||
Assay Protocol
1. Thaw and rewarm the product before use.
2. Add 1 μL phosphorylase inhibitor A solution and 1 μL phosphorylase inhibitor B solution to 100 μL lysate, mix well.
Note:
1. If the product color turns yellow, please do not use it again.
2. For your safety and health, please wear a lab coat and disposable gloves when operating.
For Research Use Only!
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