3. Haematoxylin Stain Solution; 500 mL $180

Product Information

Description

Hematoxylin is usually used in combination with eosin for staining, i.e. HE staining. Hematoxylin is oxidized to produce hematoxylin, a basic dye used in histology for staining cell nuclei. The basic principle of staining is that DNA, the main component of chromatin in the nucleus, is negatively charged and acidic, and can easily be stained by combining with the alkaline, positively charged hematoxylin dye. This hematoxylin stain can be used with eosin stain (G1001 or G1002) for HE staining of tissues or cells, and the stained nuclei are distinctly blue or blue-purple.

Storage and Transportation

Storage and transportation at room temperature, valid for 18 months.

Pre-experimental Preparation

Prepare your own hematoxylin fractionation solution (recommended G1039), hematoxylin reblue solution (recommended G1040), xylene, gradient ethanol, anhydrous ethanol, and neutral gum.

Procedure

1. Sample pre-treatment

(1) For paraffin sections: sections are dewaxed in xylene for 10 min, replaced with fresh xylene for 10 min, anhydrous ethanol for 5 min, fresh anhydrous ethanol for 5 min, 90% ethanol for 5 min, 75% ethanol for 5 min, and washed with tap water.

(2) For frozen sections: Frozen sections stored at -20°C need to be rested for 5-10 min to recover to room temperature.

2. Hematoxylin staining

The above treated sections were directly stained into hematoxylin staining solution for 3-5 min and washed with tap water; then stained by hematoxylin differentiation solution for 2-5 s and washed with tap water; hematoxylin reblue solution for 2-5 s and washed with tap water. The nuclei were observed to be blue under the microscope, and the tissue background was nearly colorless. The sections were then dehydrated sequentially by 70%, 80%, 95%, and 100% ethanol for about 3 min each. The sections were then dehydrated in fresh anhydrous ethanol for 3 min, transparent in xylene for 5 min, and replaced with fresh xylene for another 5 min. Finally, the sections were sealed with neutral gum.

Note: If used for re-staining after immunohistochemistry and other staining, please perform eosin staining after the other staining is completed.

Note

1. Hematoxylin staining solution can be reused several times and should be stored in a sealed container after each use to prevent evaporation of the active ingredients. When the tissue or cell coloring is obviously light or abnormal, please replace the staining solution with a new one.

2. A small amount of oxide film on the surface of the stain is normal and does not affect the staining result. If a large amount of oxide film appears on the surface of the staining solution, it is recommended to change to a new hematoxylin staining solution.

3. Please wear lab coat and disposable gloves during operation.

For Research Use Only!