2. Anti-Clumping Agent 100 ml

Product Information

Product Description

Most expression cell lines are domesticated to adapt to serum-free (low) and suspension culture to achieve higher cell density and increase expression yield. However, before and after domestication and in high-density suspension culture, cells often adhere to each other and clump together. Small-scale cell clusters do not affect cell growth at the beginning, but as the clusters become larger, the inner cells will be undernourished and die. The content released by the dead cells will affect the overall cell growth, leading to a larger scale of death and clumping.

This reagent is a chemically defined, protein-free and concentrated liquid reagent without any hydrolysis products, consisting of two main components: dispersant and anti-shear protectant. It provides good protection against shear while reducing intercellular adhesion and significantly improving cell clumping, resulting in a higher density of viable cells.

Storage and Shipping Conditions

Ship with wet ice; Store at 2-8°C away from light, valid for 12 months.

Product Components

Assay Protocol

Attention: The following protocol is for the addition of conventional exchange solutions, which can also be added directly to the cultured cells during use. The addition volume is the same as the following volume ratio.

1. Add the cell anti-clumping agent to the corresponding cell culture medium, with the addition volume accounting for 2% of the total volume (the amount of anti-clumping reagent required varies under different conditions, usually between 1-4%, 2% is recommended and may be adjusted.

2. Transfer the suspended cells into a centrifuge tube, centrifuge at 800 x g for 3-5 min, remove the original culture medium, and keep the cell precipitate.

3. Resuspend cells with the working solution containing the cellular anti-colonisation reagent and transfer to the appropriate culture dishes or flasks for incubation.

Note

1. This product is suitable for the domestication of suspension cell system under shaking bed culture conditions, and may not be effective for suspension cells in regular resting conditions.

2. Due to the different properties of different cells, the concentration range described in step is for reference only and can be adjusted according to specific conditions.

3. This product will affect cell transfection, it is recommended to replace it with a normal culture medium without this product before transfection incubation. Add at the time of fluid change after the transfection incubation is complete and does not affect post-transfection expression.

4. For your health and safety, please wear laboratory clothes and gloves when operating.

For Research Use Only!