14. Isopropyl β-D-1-thiogalactopyranoside (IPTG), 1g $99

Product Information

Introduction

IPTG, Isopropyl β-D-1-thiogalactopyranoside, is a placebo inducer. When X-gal is used as a chromogenic substrate, the two can be used together to screen for blue-white spots. IPTG can induce the DNA segment of the vector Lac operon to synthesize the amino-terminal fragment of β-galactosidase, which can achieve intragenic complementation (α complementation) with the defective β-galactosidase encoded by the host cell. Bacteria that achieve alpha complementation are plated on medium containing the X-gal chromogenic substrate to form blue colonies. Insertion of exogenous DNA into the multiple cloning site of the plasmid can destroy the alpha complementation, resulting in white colonies. IPTG is also a commonly used inducer of recombinant protein expression in genetic engineering.

Basic Attributes

Storage and Handling Conditions

Transported with dry ice; stored at -20°C, valid for 24 months。

Instruction

The common stock solution concentration of IPTG is 200 mg/mL. Preparation method: Accurately weigh 2 g of IPTG and dissolve it in 8 mL of distilled water. After dissolving and clarifying, dilute the volume to 10 mL, and filter and sterilize it with a 0.22 μm filter. It is recommended to divide into small portions and store at -20°C for several months. Repeated freezing and thawing should be avoided.

Note:

For your safety and health, please wear a lab coat and disposable gloves.

Note: The product may be optimized and upgraded. The actual label information prevails.

For Research Use Only!

Ver. No.: V1.0-202111