14. RPMI-1640 Medium, GlutaPlus, no Glucose, no HEPES, no Sodium Pyruvate, 500 ml $45

SKU: G4538-500ML
$45.00
In Stock
SKU: G4538-500ML Categories: , Tag:

Description

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Product Information

Product Name Cat. No. Spec.
RPMI-1640 Medium, GlutaPlus, no Glucose, no HEPES, no Sodium Pyruvate G4538-500ML 500 mL

 


Product Description/Introduction

RPMI-1640 is a cell culture medium developed by the Roswell Park Memorial Institute (RPMI). This medium was originally designed specifically for lymphocytes culture, now is widely used for various normal cell and cancer cell culture, especially for cell suspension culture, and is one of the most widely used medium. RPMI-1640 medium generally contains 11.1 mM of D-glucose, while RPMI-1640 medium without D-glucose allows the glucose concentration to be adjusted at will according to research needs.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add 5-10% serum or serum-free additives depending on the cell type.

This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 2.05 mM L-Alanyl-L-Glutamine, phenol red indicator, no D-glucose, HEPES and sodium pyruvate.

 


Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

 

For Research Use Only!

G4538 RPMI-1640 Formula

Components Concentration Components Concentration
mg/L mM mg/L mM
Amino acids Inorganic salts
Glycine 10 0.133 Calcium nitrate (Ca(NO3)2 4H2O) 100 0.4237
L-Arginine hydrochloride 241.86 1.149 Magnesium Sulfate (MgSO4) (anhyd.) 48.84 0.407
L-Asparagine hydrate 56.82 0.379 Potassium Chloride (KCl) 400 5.33
L-Aspartic Acid 20 0.15 Sodium Bicarbonate (NaHCO3) 2000 23.8
L-Cystine dihydrochloride 65.2 0.208 Sodium Chloride (NaCl) 6000 103.4
L-Glutamic Acid 20 0.136 Sodium Phosphate monobasic (NaH2PO4) (anhyd.) 800 5.63

 

L-Histidine HCl H2O 20.22 0.097 Other components
L-Hydroxyproline 20 0.153 Glutathione (reduced) 1.0 0.003
L-Isoleucine 50 0.382 Phenol Red 4.6 0.013
L-Leucine 50 0.382
L-Lysine hydrochloride 40 0.219
L-Methionine 15 0.101
L-Phenylalanine 15 0.091
L-Proline 20 0.174
L-Serine 30 0.286
L-Threonine 20 0.168
L-Tryptophan 5.0 0.025
L-Tyrosine Disodium Salt Dihydrate 28.83 0.111
L-Valine 20 0.171
L-Alanyl-L-Glutamine 446.4 2.055
Vitamins
Biotin 0.2 0.00082
Choline chloride 3.0 0.02143
D-Calcium pantothenate 0.25 0.00052
Folic Acid 1.0 0.00227
Niacinamide 1.0 0.00820
Para-Aminobenzoic Acid 1.0 0.00730
Pyridoxine hydrochloride 1.0 0.00485
Riboflavin 0.2 0.00053
Thiamine hydrochloride 1.0 0.00297
Vitamin B12 0.005 0.00000369
i-Inositol 35 0.19444

 

 

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